Isolation and Expression of Five-Amino-Acid-Deleted Variant of Feline Hepatocyte Growth Factor (HGF) cDNA
スポンサーリンク
概要
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Hepatocyte growth factor (HGF) is a pleiotropic cytokine that stimulates a wide array of cellular targets, including hepatocytes and other epithelial cells, melanocytes, endothelial and hematopoietic cells. We have cloned a different form of cDNA, with a deletion of 15 base pairs predicted to result in the loss of 5 amino acids from the first kringle domain. To investigate the biological activity, original and deleted variant of feline HGF cDNAs were transiently expressed in COS-7 cells. Both recombinant feline HGFs showed almost the same dose-response curves in the stimulation of the growth of BNL CL.2 cells (a mouse hepatocyte cell line) and scatter activity of Madin-Darby canine kidney (MDCK) cells. The findings reported here suggest that the deleted variant of feline HGF has almost the same biological activity as the original in terms of the proliferation and scatter activity.
著者
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Miyake Masashi
Central Research Laboratories Nippon Zenyaku Kogyo Co. Ltd.
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Endo Yoshio
Central Research Resource Branch Cancer Research Institute Kanazawa University
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Yamamoto Yoshimi
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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Iwabuchi Shigehiro
Central Research Laboratories Nippon Zenyaku Kogyo Co. Ltd.
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Takahashi Susumu
Department Of Applied Chemistry Graduate School Of Engineering The University Of Tokyo
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OKAZAKI Masayuki
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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YAMAMOTO Yoshimi
Department of Biochemistry and Radiation Biology, Graduate School of Veterinary Sciences, Yamaguchi University
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YAGUCHI Takahiro
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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SAZE Koh-ichi
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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WANG Janhui
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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OGAWA Toshiyuki
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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SUZUTA Yasuyuki
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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HAGA Yoshihisa
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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WAKI Takashi
Department of Biochemistry and Radiation Biology, Graduate School of Veterinary Sciences, Yamaguchi University
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MIYAKE Masashi
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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ENDO Yoshio
Central Research Laboratories, Nippon Zenyaku Kogyo Co., Ltd.
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