Structure Elucidation of Major Metabolites from Medroxyprogesterone Acetate by P450
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概要
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We previously reported the separation and identification for the major metabolites from incubating medroxyprogesterone acetate (MPA) with P450. The structure assignments for these metabolites were tentatively assigned based on one-dimensional (1D) proton NMR. Unambiguous structure identification of these metabolites is critical to study biological pathways of P450. Here we report the complete structure elucidation by extensive two-dimensional (2D) NMR for the three major metabolites isolated in our earlier studies. The three major metabolites (namely M-2, M-3, M-4) were unambiguously identified to be 6β-, 1β-, and 2β-hydroxy MPA. The current work confirmed the speculated structures for these metabolites in our previous studies. More importantly, the unambiguous structural information and the establishment for their NMR chemical shifts of these metabolites can serve as reference standards for future studies.
- 公益社団法人 日本薬学会の論文
著者
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LI Wei
Department of Hematology, Affiliated Hospital of Academy of Military Medicine Science
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YANG Ling
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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Liu Yong
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Yang Ling
Laboratory Of Pharmaceutical Resource Discovery Biotechnology Division Dalian Institute Of Chemical
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Chen Jianjun
Department of Pharmaceutical Sciences, College of Pharmacy, The University of Tennessee Health Scien
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Zhang Jiang-Wei
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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Li Wei
Department Of Chinese Material Medicine Chinese Material Medicine College Of Jilin Agricultural Univ
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Yang Ling
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Academy of Sciences
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Li Wei
Department of Pharmaceutical Sciences, College of Pharmacy, The University of Tennessee Health Science Center
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Chen Jianjun
Department of Pharmaceutical Sciences, College of Pharmacy, The University of Tennessee Health Science Center
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