Acceptor Specificity and Transfer Efficiency of a β-Glycosidase from the China White Jade Snail

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概要

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• The acceptor specificity and transfer potential of a β-D-glycosidase (G I), which had been purified from the China white jade snail, were further investigated by using various sugars as acceptors. G I had broad monosaccharide acceptor specificity for its transglycosylation activity. More specifically, it efficiently catalyzed the transfer of the β-D-fucosyl, β-D-glucosyl or β-D-galactosyl moiety from the corresponding p-nitrophenyl-β-D-glycopyranosides to various monosaccharides. The transfucosylation efficiency of G I was studied by using p-nitrophenyl-β-D-fucopyranoside (pNPFuc) as the donor and glucose and xylose as the acceptors. The yields under conditions of non-initial velocity were 88% for glucose and 93% for xylose. The transfer product with glucose as the acceptor was isolated and identified as β-fucosyl-1,6-glucose by an NMR analysis. The data from these analyses indicate that G I had broad acceptor specificity and high efficiency for transglycosylation. These uncommon properties of G I could make it a valuable biocatalyst for the synthesis of various disaccharides.

• 社団法人 日本農芸化学会の論文

著者

• LIU Yong

  Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade

• YANG Ling

  Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade

• Hu Ying

  Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade

• Luan Hongwei

  Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade

• Liu Huixin

  Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade

• GE Guangbo

  Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade

• ZHOU Kun

  Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade

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