Acceptor Specificity and Transfer Efficiency of a β-Glycosidase from the China White Jade Snail
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概要
- 論文の詳細を見る
The acceptor specificity and transfer potential of a β-D-glycosidase (G I), which had been purified from the China white jade snail, were further investigated by using various sugars as acceptors. G I had broad monosaccharide acceptor specificity for its transglycosylation activity. More specifically, it efficiently catalyzed the transfer of the β-D-fucosyl, β-D-glucosyl or β-D-galactosyl moiety from the corresponding p-nitrophenyl-β-D-glycopyranosides to various monosaccharides. The transfucosylation efficiency of G I was studied by using p-nitrophenyl-β-D-fucopyranoside (pNPFuc) as the donor and glucose and xylose as the acceptors. The yields under conditions of non-initial velocity were 88% for glucose and 93% for xylose. The transfer product with glucose as the acceptor was isolated and identified as β-fucosyl-1,6-glucose by an NMR analysis. The data from these analyses indicate that G I had broad acceptor specificity and high efficiency for transglycosylation. These uncommon properties of G I could make it a valuable biocatalyst for the synthesis of various disaccharides.
- 社団法人 日本農芸化学会の論文
- 2009-03-23
著者
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LIU Yong
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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YANG Ling
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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Liu Yong
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Yang Ling
Laboratory Of Pharmaceutical Resource Discovery Biotechnology Division Dalian Institute Of Chemical
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Hu Ying
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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Luan Hongwei
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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Liu Huixin
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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GE Guangbo
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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ZHOU Kun
Laboratory of Pharmaceutical Resource Discovery, Dalian Institute of Chemical Physics, Chinese Acade
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Yang Ling
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Luan Hongwei
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Hu Ying
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Liu Huixin
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Liu Yong
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Zhou Kun
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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Ge Guangbo
Laboratory Of Pharmaceutical Resource Discovery Dalian Institute Of Chemical Physics Chinese Academy
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