Identification of High γ-Aminobutyric Acid Producing Marine Yeast Strains by Physiological and Biochemical Characteristics and Gene Sequence Analyses
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概要
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Four marine yeasts isolated from the Pacific Ocean off Japan (Siki No. 4, Siki No. 15, Hach No. 6, and Inub No. 11), which showed high γ-aminobutyric acid (GABA) producing abilities, were identified and classified by physiological and biochemical characteristics and gene sequence analyses. Analysis of biochemical data suggested that while Siki No. 15 was identical to Candida, the remaining three isolates belonged to the genus Pichia. However, these data were insufficient to resolve their identity at the species level. Subsequently, analysis of the 5.8S rRNA genes and the two internal transcribed spacer regions (ITS) sequences revealed that Siki No. 15 belongs to Pichia guilliermondii, while the remaining three isolates corresponded to Pichia anomala. Since Siki No. 4 showed slightly different biochemical properties than the other two isolates, which were otherwise identical, we sought to investigate the sequences of the intergenic spacer region 1 (IGS1). We observed few nucleotide changes, suggesting that the Hach No. 6 and Inub No. 11 isolates belong to different but new strains for which we propose the names P. anomola MR-1 and MR-2 respectively.
- 社団法人 日本農芸化学会の論文
著者
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WATABE Shugo
Laboratory of Aquatic Molecular Biology and Biotechnology, Graduate School of Agricultural and Life
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GUO Xiao-feng
Research and Development Division, Nichirei Foods Inc.
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AOKI Hitoshi
Research and Development Division, Nichirei Foods Inc.
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HAGIWARA Toshihiko
Research and Development Division, Nichirei Foods Inc.
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MASUDA Kazuaki
Research and Development Division, Nichirei Foods Inc.
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Guo Xiao-feng
Research And Development Division Nichirei Foods Inc.
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