Crystal structure of the short-chain flavin reductase HpaC from Sulfolobus tokodaii strain 7
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概要
- 論文の詳細を見る
Flavin reductases (FRs) catalyze the reduction of free flavins using NADH or NADPH. In recent years, a new family of short-chain FRs have been identified in a variety of bacteria and archaea. Here we have determined the crystal structure of an archaeal short-chain FR, HpaC from an aerobic thermoacidophilic crenarchaeon Sulfolobus tokodaii strain 7. The HpaC molecule exists as a homodimer with one FMN for each monomer. On the other hand, PheA2, the most structurally similar protein to HpaC, contains FAD rather than FMN. Structural comparison of these proteins has revealed that the short loop at residues 82-83 and the successive η1 helix in HpaC are closer to the bound flavin than those in PheA2. As a result, there is not sufficient space to accommodate the AMP moiety of FAD in HpaC, and thus HpaC prefers FMN to FAD. (Communicated by Masanori OTSUKA, M.J.A.)
- 日本学士院の論文
著者
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Lee Woo
Graduate School Of Pharmaceutical Sciences The University Of Tokushima
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Tanokura Masaru
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Nagata Koji
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
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Kudo Norio
Department Of Physiology Institute Of Basic Medical Sciences University Of Tsukuba
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Kudo Norio
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Kudo Norio
Biotechnology Research Center The University Of Tokyo
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Nagata Koji
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni
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Nagata Kazuhiro
Depatrment Of Molecular And Cellular Biology Institute For Frontier Medical Sciences Kyoto Universit
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NAGATA Koji
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Kudo N
Department Of Physiology Institute Of Basic Medical Sciences University Of Tsukuba
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Okai Masahiko
Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers
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OKAI Masahiko
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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KUDO Norio
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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KAMO Masayuki
Graduate School of Agricultural and Life Sciences, The University of Tokyo
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Nagata Koji
Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences The Univ
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Nagata Koji
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Lee Woo
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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