Crystal structure of monomeric sarcosine oxidase from Bacillus sp. NS-129 reveals multiple conformations at the active-site loop

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概要

• 論文の詳細を見る

• Monomeric sarcosine oxidase (MSOX) is a flavoprotein that catalyzes the oxidation of sarcosine to generate formaldehyde, glycine, and hydrogen peroxide, and is utilized in quantification of creatinine in serum. Crystal structure of MSOX from Bacillus sp. NS-129 (without ligand) has been determined at 1.86 Å Unlike the published structures of MSOX from Bacillus sp. B-0618 (without or with carboxylate-containing ligand), the two molecules in the asymmetric unit adopt distinct conformations at the active site loop (Gly56 to Glu60) with a maximal root-mean-square (RMS) displacement of 3.3 Å for Cα atom of Arg59. The multiple conformations seen at the active-site loop suggest that high flexibility of the loop would be important for the activity of MSOX. (Communicated by Masanori OTSUKA, M.J.A.)

• 日本学士院の論文

著者

• Tanokura Masaru

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Kubota Keiko

  Graduate School of Science, Hiroshima University

• Kubota Keiko

  Graduate School Of Science Hiroshima University

• Nagata Koji

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences Univers

• Ohtsuka Jun

  Dept. of Appl. Biol. Chem. Grad. Sch. of Agr. and Life Sci. Univ. of Tokyo

• Nagata Koji

  Department Of Applied Biological Chemistry Graduate School Of Agricultural And Life Sciences The Uni

• ICHIKAWA Toshio

  Research and Development Division, Kikkoman Corporation

• Nagata Kazuhiro

  Depatrment Of Molecular And Cellular Biology Institute For Frontier Medical Sciences Kyoto Universit

• NAGATA Koji

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• SASAKI Hiroshi

  Graduate School of Science and Engineering, Saga University

• OKAI Masahiko

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• KAMO Masayuki

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• HUA Ming

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• ITO Kosuke

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Nagata Koji

  Department Of Applied Biological Chemistry Graduate School Of Agriculture And Life Sciences The Univ

• Ichikawa Toshio

  Research And Development Division Kikkoman Corporation

• Nagata Koji

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Sasaki Hiroshi

  Graduate School Of Science And Engineering Saga University

• Sasaki Hiroshi

  Graduate School of Information Science and Technology, The University of Tokyo

• KUBOTA Keiko

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Ohtsuka Jun

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• SASAKI Hiroshi

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

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