The Dismutation of Aldehydes by a Bacterial Enzyme
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概要
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Formaldehyde-resistant Pseudomonas putida F61 isolated from a soil sample showed high activities of formaldehyde dehydrogenase (EC 1.2.1.1) and a specific enzyme catalyzing the dismutation of formaldehyde to form methanol and formate. The latter enzyme, given the trivial name of formaldehyde dismutase, was purified to electrophoretic homogeneity from a cell-free extract of P. putida F61. The enzyme was a tetramer with a molecular weight of approximately 2.2 x 105, and an isoelectric point of 4.8. The enzyme catalyzed the stoichiometric dismutation of formaldehyde and acetaldehyde to form a half mol of each of the corresponding alcohol and acid without addition of an electron acceptor, but it did not catalyze the dismutation of propionaldehyde, butyraldehyde and so on. The apparent Km for formaldehyde was found to be 350mM. One of the most unique properties of the enzyme was the catalytic activity of crossdismutation between two different aldehydes, such as formaldehyde/acetaldehyde, formaldehyde/propionaldehyde, and so on.
- 社団法人 日本農芸化学会の論文
著者
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SAKAZAWA CHIKAHIRO
Departments of Biotechnology Faculty of Engineering Tottori University
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Kato Nobuo
Department Of Agricultural Chemistry Faculty Of Agriculture Kyoto University
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Kobayashi Hisataka
Department Of Diagnostic And Interventional Imageology Graduate School Of Medicine Kyoto University
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SHIRAKAWA Kamon
Department of Environmental Chemistry and Technology, Tottori University
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