Microbial Oxidation of Isoprenoid Alkanes, Phytane, Norpristane and Farnesane

概要

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Rhodococcus sp. BPM 1613, a pristane oxidizing microorganism, grows on isoprenoid hydrocarbons such as phytane (2, 6, 10, 14-tetramethylhexadecane), norpristane (2, 6, 10-trimethylpentadecane) and farnesane (2, 6, 10-trimethyldodecane) as the sole carbon source, resulting in accumulation of oxidation products in the culture broth. The oxidation products of phytane, norpristane and farnesane in the respective culture broth were isolated and purified by the use of silica gel column chromatography. Their chemical structures were determined by instrumental analyses such as IR, NMR and mass spectrometry. The oxidation products of phytane were identified as 2, 6, 10, 14-tetramethyl-1-hexadecanol and 2, 6, 10, 14-tetramethylhexadecanoic acid, the product of norpristane as 2, 6, 10-trimethyl-l-pentadecanol, and that of farnesane as 2, 6, 10-trimethyl-1-dodecanol. All these oxidation products were either monoalcohols or monocarboxylic acids derived through oxidation of the isopropyl terminus of each alkane. In addition, the relationship between the terminal structure of isoprenoid hydrocarbons and microbial oxidation was explored on the basis of these results.
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