Investigation of Glucose 6-Phosphate Production and Simultaneous Carboxy-Terminated PAMAM-Bound Adenine Nucleotides Regeneration by Conjugated Enzymes in a Batch Reactor

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The production of glucose 6-phosphate (G6P) and simultaneous carboxy-terminated PAMAM-bound adenine nucleotides regeneration by the conjugated enzymes, Bacillus stearothermophilus glucokinase (GK) and acetate kinase (AK), in a small batch reactor were investigated both theoretically and experimentally. As the cofactor, a new family of polymer-bound adenine nucleotides (ATP and ADP) was used which was prepared in our previous work (Abdelmoez et al., 2002) by binding native ATP or ADP to a carboxy-terminated polyamidoamine (PAMAM) dendrimer (polymer-bound ATP or ADP). The experimental time courses of the glucose conversion were in a good agreement with the theoretical predictions calculated using the rate equations of G6P synthesis catalyzed by B. stearothermophilus GK (Abdelmoez et al., 2004) and the simplified rate equation of polymer-bound ATP regeneration catalyzed by B. stearothermophilus (AK) which is proposed in the present work.
社団法人化学工学会の論文
2004-11-01