キチンおよびキトサン分解性細菌を利用したバラうどんこ病菌の生物防除

概要

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[Synopsis] In this study, we attempted to isolate Chitin- and Chitosan-degrading bacteria from rose phylloplane for their use in biological control of rose powdery mildew pathogen, Sphaerotheca pannosa. Rose leaves collected from rose garden were stamped on M9 medium containing colloidal chitin or colloidal chitosan for short period of time and removed. The culture plates were incubated at 26℃ for 3days and colonies grew and formed a halo on the medium were isolated as candidates for chitin and/or chitosan degrading bacteria. Two isolates, KYT-001 and KYT-002 were found to extensively degrade chitin and chitosan on the basis of the size of haloes on the plates. In view of our previouse finding that commercially available chitinase degrade conidia and haustoria of the pathogen, both the isolates were tested for their enzyme activities. Prior to the assessment of their control activity of the disease, KYT-001 and KYT-002 were inoculated on rose phylloplane, incubated at 25℃ for certain period of time and isolated. These bacteria were found to be capable of surviving on rose phylloplane for a long period of time. These results indicated that chitin- and chitosan-degrading bacteria isolated in this study could be useful for biological control of powdery mildew pathogen.
1999-03-00