パーティクルガン法を利用したオオムギ子葉鞘細胞およびオオムギうどんこ病菌への外来遺伝子の導入

概要

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[Synopsis] In this study, we established a gene delivery system for Biumeria graminis f. sp. hordei by microprojectile bombardment. Tungsten particles were coated with the plasmid pBI/s-GFP which had been constructed from pBI121 by replacing the GUS gene with s-GFP. The coated plasmid was introduced into barley coleoptile epidermal cells or conidiospores of B. graminis f. sp. hordei by microprojectile bombardment. The expression of s-GFP gene in transformed coleoptile cells and conidiospores were detected by green fluorescence of GFP under the fluorescent microscope. The fluorescent condiospores were transferred by micromanipulation to non-inoculated coleoptile tissues to differentiated from non-transformed conidiospores. The transferred conidiospores germinated, invaded after appressorium formation, formed haustria and secondary hyphae, indicating that the gene delivery system established in this study is useful for introducing foreign genes not only into barley coleoptile epidermal cells and B. graminis f. sp. hordei, but also other obligately parasitic disease complexes, because easy detection of introduced gene expression in the transformed cells.
近畿大学農学総合研究所の論文
2000-03-00