Changes in plasma gonadotropins, inhibin and testosterone concentrations and testicular gonadotropin receptor mRNA expression during testicular active, regressive and recrudescent phase in the captive Japanese black bear (Ursus thibetanus japonicus)
スポンサーリンク
概要
- 論文の詳細を見る
Male Japanese black bears (Ursus thibetanus japonicus) have an explicit reproductive cycle. The objective of this study was to clarify the variation of plasma testosterone, FSH, inhibin, LH levels and testicular gonadotropin receptor mRNA expression of male bears associated with their testicular activity. Notably, this study investigated peripheral FSH concentration and localization of gonadotropin receptor mRNAs for the first time in male bears. Blood and testicular tissue samples were taken from captive, mature, male Japanese black bears during testicular active, regressive and recrudescent phases. Plasma hormone concentrations were measured by immunoassays, and gonadotropin receptor mRNA expression in the testis was investigated by in situ hybridization technique and also by real-time PCR. There were significant variations in plasma testosterone and inhibin concentrations. Changes in FSH concentration preceded these hormones with a similar tendency. Hormones started to increase during denning, and achieved the highest values at the end of the recrudescent phase for FSH and in the active phase for testosterone and inhibin. These changes in hormone concentrations were accompanied by testicular growth. In situ hybridization analysis revealed that FSH and LH receptor mRNA was possibly expressed in Sertoli cells and Leydig cells, respectively, as they are in other mammals. However, neither plasma LH concentration nor testicular gonadotropin receptor mRNA expression level varied significantly among the sampling months. These results suggest that FSH, inhibin and testosterone have roles in testicular activity in male bears. This study provides important endocrine information for comprehending seasonal reproductivity in male Japanese black bears.
著者
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坪田 敏男
北海道大学大学院獣医学研究科生態学教室
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Watanabe Gen
Lab. Of Veterinary Physiology Dep. Of Veterinary Medicine Fac. Of Agriculture Tokyo Univ. Of Agricul
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Watanabe Gen
岐阜大学 連合獣医学研究科基礎獣医学
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Taya Kazuyoshi
岐阜大学 連合獣医学研究科基礎獣医学
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Taya K
Tokyo Univ. Agriculture And Technol. Tokyo
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Taya Kazuyoshi
日本中央競馬会競走馬総合研究所
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坪田 敏男
岐阜大学農学部家畜臨床繁殖学講座
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TSUBOTA Toshio
岐阜大学農学部
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IIBUCHI Ruriko
Laboratory of Wildlife Biology, Graduate School of Veterinary Medicine, Hokkaido University
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Watanabe G
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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Taya K
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Faculty Of Agriculture Tokyo U
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Taya K
Dep. Of Basic Veterinary Sci. The United Graduate School Of Veterinary Sciences Gifu Univ.
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坪田 敏男
兵庫県森林動物研究センター
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坪田 敏男
岐阜大学 農
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坪田 敏男
岐阜大学農学部
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Tsubota Toshio
Laboratory Of Wildlife Biology Graduate School Of Veterinary Medicine Hokkaido University
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Tsubota Toshio
Gifu Univ. Gifu Jpn
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Kamine Akari
Laboratory Of Wildlife Biology Graduate School Of Veterinary Medicine Hokkaido University
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Taya Kazuyoshi
中華人民共和国
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坪田 敏男
北海道大学大学院獣医学研究科
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Taya Kazuyoshi
Laboratory Of Veterinary Physiology Tokyo University Of Agriculture And Technology
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Taya K
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Tokyo University Of Agricultur
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Tsubota Toshio
Department Of Environmental Veterinary Science Graduate School Of Veterinary Medicine Hokkaido Unive
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Iibuchi Ruriko
Laboratory Of Wildlife Biology Graduate School Of Veterinary Medicine Hokkaido University
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Nio-kobayashi Junko
Lab. Of Histology And Cytology Hokkaido Univ. Graduate School Of Medicine
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Tsubota T
Department Of Environmental Veterinary Science Graduate School Of Veterinary Medicine Hokkaido Unive
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Tsubota Toshio
Laboratory Of Ecology Department Of Environmental Veterinary Sciences Graduate School Of Veterinary
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Tsubota Toshio
Faculty Of Agriculture Gifu University
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Tsubota Toshio
Division Of Veterinary Medicine Faculty Of Agriculture Gifu University
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Tsubota Toshio
Laboratory Of Theriogenology Faculty Of Agriculture Gifu University
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Tsubota Toshio
Department Of Theriogenology Faculty Of Agriculture Gifu University
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Tsubota Toshio
United Graduate School Of Veterinary Medicine Gifu University
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坪田 敏男
岐阜大学農学部獣医学科 : 岐阜大学大学院連合獣医学研究科
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Shimozuru Michito
Laboratory Of Wildlife Biology Graduate School Of Veterinary Medicine Hokkaido University
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坪田 敏男
岐阜大学 大学院連合獣医学研究科
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Taya Kazuyoshi
Laboratory Of Veterinary Physiology Department Of Veterinary Medicine Tokyo University Of Agricultur
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Shimozuru Michito
Laboratory Of Veterinary Ethology The University Of Tokyo
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Watanabe Gen
中華人民共和国
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Taya Kazuyoshi
東京農工大学 農学部獣医学科獣医生理学
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Tsubota Toshio
Department Of Veterinary Obstetrics Faculty Of Veterinary Medicine Hokkaido University
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Takano Kaoru
Laboratory Of Experimental Animal Models Division Of Bioresources National Institute Of Biomedical I
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Tsubota Toshio
Department Of Basic Veterinary Science The United Graduate School Of Veterinary Sciences Gifu Univer
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Takimoto Kazuhiro
Department Of Veterinary Science National Institute Of Health
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Tsubota Toshio
Laboratory Of Zoo And Wildlife Medicine Gifu University
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Tago Kazumi
Hatano Research Institute Food And Drug Safety Center
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SHIMOZURU Michito
Laboratory of Wildlife Biology and Medicine, Graduate School of Veterinary Medicine, Hokkaido University
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