Establishment of Tissue Culture System of Chinese Poplars : Populus tomentosa and Populus alba cv. Pyramidalis×Populus tomentosa

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概要

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• 中国産ポプラの毛白楊（Populus tomentosa）と毛新楊（P. alba cv. Pyramidalis×P. tomentosa）の芽生えを外植体とした組織培養系を確立した。発芽後の子葉と胚軸を切り出し，BAP（0.4，0.8mg/l）を添加した1/2MS培地上でマルチプルシュートを得た。伸長したシュートをホルモンフリーの1/2MS培地に移植し，発根を誘導し幼植物体を得た。これをピートモスとバーミキュライトを用土として順化させた。またシュートは初代培養と同じ培地を用いて継代培養することができた。For the improvement of poplars which are resistant to environmental stresses, and for conservation of genotypes, we established the protocols for inducing organogenesis in vitro. And plantlets were regenerated from explants obtained from young seedlings of Populus tomentosa and P. alba cv. Pyramidalis×P. tomentosa. Hypocotyls with cotyledon were excised from 5-day-old seedlings and were surface sterilized. Explants formed multiple shoots on 1/2 MS medium containing BAP (0.4, 0.8mg/l). Elongated shoots were transferred to hormone-free 1/2 MS medium and roots were induced on it. Regenerated plantlets were acclimatized in a mixture of peat moss and vermiculite under controlled relative humidity during 3 weeks. Subculture of multiple shoot was possible by plantation of shoots on the same medium of the first culture.

• 東京大学大学院農学生命科学研究科附属演習林,The Tokyo University Forests,Department of Forestry, Faculty of Agriculture, The University of Tokyo,Research Division of the University Forests, Faculty of Agriculture, The University of Tokyoの論文

著者

• Kang J‐m

  Univ. Tokyo Tokyo Jpn

• Kang Jae-myung

  Faculty Of Agriculture The University Of Tokyo

• Kojima Katsumi

  Asian Natural Environmental Science Center The University Of Tokyo

• Kojima Kenji

  Asian Natural Environmental Science Center The University Of Tokyo

• Ide Y

  Univ. Tokyo

• Ide Yuji

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Ide Yuji

  Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology

• Ide Yuji

  Laboratory Of Forest Ecosystem Studies Department Of Ecosystem Studies Graduate School Of Agricultur

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