Isolation of Protoplasts from Various Tissues of Acacia mangium Cultured in vitro

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概要

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• Various enzyme combinations were tested for protoplast isolation from compound leaves of in vitro cultured shoots of Acacia mangium. The combination of 1% Cellulase 'ONOZUKA'RS, 0.2% Pectolyase Y-23 and 2% Driselase was optimal to obtain a high yields of protoplasts. The protoplasts were also successfully isolated from compound leaves and cotyledons of young seedlings and callus, but not from phyllode.アカシアマンギウムの培養シュートの本葉からプロトプラストを単離する酵素組成を検索した。酵素液は，セルラーゼ“オノズカ”RS1％，ペクトリアーゼY-23，0.2％，ドリセラーゼ2％の添加が適当と判断された。また，芽生えの本葉，子葉およびカルスからプロトプラストを単離できたが，仮葉からは単離できなかった。

• 東京大学大学院農学生命科学研究科附属演習林,The Tokyo University Forests,Department of Forestry, Faculty of Agriculture, The University of Tokyo,Research Division of the University Forests, Faculty of Agriculture, The University of Tokyoの論文

著者

• Kojima Katsumi

  Asian Natural Environmental Science Center The University Of Tokyo

• sasaki Satohiko

  College of Bioresource Sciences, Nihon University

• Sasaki Satohiko

  College Of Bioresource Sciences Nihon University

• Kojima Kenji

  Asian Natural Environmental Science Center The University Of Tokyo

• Ide Y

  Univ. Tokyo

• Ide Yuji

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

• Ide Yuji

  Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology

• Ide Yuji

  Laboratory Of Forest Ecosystem Studies Department Of Ecosystem Studies Graduate School Of Agricultur

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