Establishment of a Callus Culture System of Populus euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii × Populus plantierensis
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Populus euphratica（コトカケヤナギ，中国名，胡楊），Populus alba cv. Pyramidalis（中国名，新疆楊），Populus maximowiczii×Populus plantierensis（品種名，FS－51）の3種類のポプラについてカルス培養の基本的手法を確立した。BAP，NAA，2，4－Dを単独あるいは組み合わせて添加したMS培地上でカルス誘導が可能であった。誘導されたカルスは，BAPを0.2mg/l，NAAを1.0mg/l含むMS培地上で継代培養可能であった。また，継代2回目のカルスをBAPを単独あるいはNAAまたはGA3を組み合わせたMS培地上でシュートが再生した。これらのシュートは，1/2MS培地にさしつけることにより発根して植物体が再生した。三種類すべてでカルスの培養と植物体の再生が可能であったが，種によって適当なホルモン条件は異なっていた。A basic protocol of callus culture of Populus. euphratica, Populus alba cv. Pyramidalis and Populus maximowiczii × Populus plantierensis (FS-51) was established. Callus was induced on MS medium containing BAP, NAA and 2,4-D in combination or alone. The callus could be subcultured on MS medium supplemented with 0.2mg/l BAP and 1.0mg/l NAA. When callus was cultured on the medium containing BAP in combination with NAA or GA3, adventitious shoots were regenerated. The shoots were successfully rooted on 1/2 MS medium. Callus culture and plant regeneration were possible in all species tested. However, there were some differences in the hormonal condition suitable for callus formation and shoot regeneration among species.
- 東京大学大学院農学生命科学研究科附属演習林,The Tokyo University Forests,東京大学農学部附属演習林研究部,中国東北林業大学,東京大学大学院農学生命科学研究科,東京大学農学部附属演習林千葉演習林,Research Division of the University Forests, Faculty of Agriculture, The University of Tokyo,Faculty of Forest Resources and Environment, Northeの論文
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