Isolation of Template Active RNA from the Pine Wood Nematodes, Bursaphelenchus xylophilus

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概要

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• マツノザイセンチュウ（Bursaphelenchus xylophilus）からSDS/フェノール・塩化セシウム法によりRNAを抽出し，ポリ（A）＋RNAを精製した．さらにこのマツノザイセンチュウに由来するポリ（A）＋RNAが鋳型活性を持っことを確かめた．これを用いてマツノザイセンチュウ病の病原物質の候補であるセルラーゼの起源が証明される可能性がある．Total RNA was successufully isolated from the pine wood nematodes, Bursaphelenchus xylophilus, by the SDS-phenol/CsCl method. Poly(A)+ RNA was purified from the RNA, and was shown to have template activity. Since poly(A)+ RNA is produced by eucaryotes, including the pine wood nematode, the template active poly(A)+ RNA of the pine wood nematodes can be used to clarify the origin of cellulase in the pine wilt disease.

• 東京大学大学院農学生命科学研究科附属演習林,The Tokyo University Forests,Department of Forestry, Faculty of Agriculture, The University of Tokyo,東京大学農学部林学科の論文

著者

• Kojima Katsumi

  Asian Natural Environmental Science Center The University Of Tokyo

• sasaki Satohiko

  College of Bioresource Sciences, Nihon University

• Sasaki Satohiko

  College Of Bioresource Sciences Nihon University

• Kojima Kenji

  Asian Natural Environmental Science Center The University Of Tokyo

• MASUMORI Masaya

  Graduate School of Agricultural and Life Sciences, The University of Tokyo

• Masumori M

  Graduate School Of Agricultural And Life Sciences The University Of Tokyo

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