Isolation and Culture of Leaf Protoplasts from In Vitro Subcultured Poplars: Populus tomentosa, Populus alba cv. Pyramidalis×Populus tomentosa and Populus maximowiczii×Populus plantierensis
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概要
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Leaf protolasts were successfully isolated from in vitro cultured plantlets of Populus tomentosa, Populus alba cv. Pyramidalis×Populus tomentosa and Populus maximowiczii×Populus plantierensis by using enzyme solution containing 1% Cellulase 'Onozuka'RS and 0.25% Pectolyase Y-23 in 0.6M mannitol solution. Cell division and cell cluster formation were observed in P. maximowiczii×P. plantierensis during successive culture in a modified MS liquid medium containing BAP and 2,4-D, from which ammonium nitrate was eliminated.試験管内の継代培養により育成した3種類のポプラ（Populus tomentosa, Populus alba cv. Pyramidalis×Populus tomentosa, Populus maximowiczii×Populus plantierensis）の幼植物体の葉を，1％のセルラーゼ‘オノヅカ’RSと0.25％のペクトリアーゼY-23，0.6Mマニトールを含む酵素液で処理し，プロトプラストを得ることが出来た。このプロトプラストを硝酸アンモニウムを除いたMS培地にBAPと2,4-Dを添加して培養したところ，P. maximowiczii×P. plantierensisで細胞分裂が観察され，引き続いて，細胞集塊の形成が認められた。
- 東京大学大学院農学生命科学研究科附属演習林,The Tokyo University Forests,東京大学農学部林学科,東京大学農学部附属演習林研究部,Department of Forestry, Faculty of Agriculture, The University of Tokyo,Research Division of the University Forests, Faculty of Agriculture, The University of Tokyoの論文
著者
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Kang J‐m
Univ. Tokyo Tokyo Jpn
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Kang Jae-myung
Faculty Of Agriculture The University Of Tokyo
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Sasaki Satohiko
College Of Bioresource Sciences Nihon University
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Ide Yuji
Graduate School Of Agricultural And Life Sciences The University Of Tokyo
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Ide Yuji
Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology
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Ide Yuji
Laboratory Of Forest Ecosystem Studies Department Of Ecosystem Studies Graduate School Of Agricultur
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