植物の低温下における遺伝子発現の生体内解析システムの開発

概要

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Hundreds of genes are known to respond to cold acclimation and the quantitative changes of their gene products have been investigated over many years. However, only a small number of cold-responsive genes have been subjected to the thorough analysis, and little is known about their spatial distribution and temporal regulation in planta during exposure to low temperature. Using luciferase as the reporter gene, we developed in vivo imaging and analysis method of individual plants under controlled environment to evaluate where and when the promoter is regulated. Analysis of the promoter of COR15A, a well-known cold-responsive gene, showed distinctive temporal patterns in responses to various temperatures, and the rate of its induction was highly dependent on the temperature. Moreover, the expression of COR15A promoter::ELuc corresponded to the photoperiods, indicating that the cold-inducible genes are regulated by a complex interaction of multiple environmental stimuli.
低温生物工学会の論文
2013-10-15