Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
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概要
- 論文の詳細を見る
Hyaluronan (HA) synthesis by microalgal Chlorella cells in combination with chloroviruses represents a unique ecofriendly process for converting solar energy and CO_2 into useful materials. However, at the final stage of viral infection, infected host cells are completely lysed, and thus HA should be harvested before cell lysis. In the current study, two methods were investigated to improve the yield of HA: (i) adopting slow-growing chlorovirus isolates and (ii) modification of the virus replication process using an inhibitor of DNA synthesis, aphidicolin. Compared with Paramecium bursaria Chlorella virus type I (PBCV-1), the prototype chlorovirus, slow-growing virus isolates (CVO1 and CVTS1) produced a 1.5 times higher concentration of HA in infected Chlorella cultures. Furthermore, addition of aphidicolin, an inhibitor of DNA synthesis, delayed virus replication and increased the final concentration of HA 1.5-fold that of cultures without the addition of aphidicolin. Therefore, a 2-to 3-fold increase in the yield of HA by the Chlorella-virus system was attained by using slow-growing viral isolates and the addition of aphidicolin.
- 2013-05-00
著者
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Kawasaki Takeru
Department Of Molecular Biotechnology Graduate School Of Advanced Matter Hiroshima University
-
Fujie Makoto
Department Of Fermentation Technology Faculty Of Engineering Hiroshima University
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Yamada Takashi
Department Of Animal Science Graduate School Of Agricultural Science Kobe University
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Kawasaki Takeru
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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Rakkhumkaew Numfon
Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
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