A simple fed-batch method for transcription and insect cell-free translation(METHODS)
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概要
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We tested a one batch reaction method for the transcription and the insect cell-free translation from undigested plasmids without any centrifugation steps. The efficiency of protein synthesis reached 74-112% of that achieved using the conventional procedure. This simplified method will help expedite the high throughput insect cell-free protein production.
- 2012-12-00
著者
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Uozumi Nobuyuki
Department Of Biomolecular Engineering Graduate School Of Engineering Tohoku University
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Ezure Toru
Clinical and Biotechnology Business Unit, Life Science Business Department, Analytical and Measuring Instruments Division, Shimadzu Corporation
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Sato Yoko
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Aizawa Keishi
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Ando Eiji
Clinical and Biotechnology Business Unit, Life Science Business Department, Analytical and Measuring Instruments Division, Shimadzu Corporation
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