Gene Suppression of Mouse Testis In Vivo Using Small Interfering RNA Derived from Plasmid Vectors
スポンサーリンク
概要
- 論文の詳細を見る
We evaluated whether inhibiting gene expression by small interfering RNA (siRNA) can be used for an in vivo model using a germ cell-specific gene (Tex101) as a model target in mouse testis. We generated plasmid-based expression vectors of siRNA targeting the Tex101 gene and transfected them into postnatal day 10 mouse testes by in vivo electroporation. After optimizing the electroporation conditions using a vector transfected into the mouse testis, a combination of high- and low-voltage pulses showed excellent transfection efficiency for the vectors with minimal tissue damage, but gene suppression was transient. Gene suppression by in vivo electroporation may be helpful as an alternative approach when designing experiments to unravel the basic role of testicular molecules.
著者
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Ishikawa Tomoko
Department Of Anatomy Nippon Medical School
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Araki Yoshihiko
Institute For Environmental And Gender-specific Medicine Juntendo University Graduate School Of Medi
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Koji Takehiko
Department Fo Histology And Cell Biology Nagasaki University School Of Medicine
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Takizawa Toshihiro
Department Of Anatomy Jichi Medical School
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Takizawa Takami
Department of Anatomy, Jichi Medical School
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Mizuguchi Yoshiaki
Department Of Surgery Nippon Medical School
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Sato Yoko
Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University
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Ishikawa Tomoko
Department of Molecular Medicine and Anatomy, Nippon Medical School
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Kosuge Takuji
Department of Molecular Medicine and Anatomy, Nippon Medical School
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Takizawa Toshihiro
Department of Molecular Medicine and Anatomy, Nippon Medical School
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Mizuguchi Yoshiaki
Department of Surgery for Organ Function and Biological Regulation, Nippon Medical School
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Takizawa Takami
Department of Molecular Medicine and Anatomy, Nippon Medical School
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