Antigen Retrieval on Ultrathin Cryosections(INVITED ARTICLE)
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概要
- 論文の詳細を見る
We have studied two different monoclonal antibodies that behave poorly for the immunocytochemical localization of caveolin-1 and caveolin-2 in paraformaldehyde fixed tissue. Treatment of tissue sections with sodium dodecyl sulfate (SDS) led to enhanced immunocytochemical detection of antigens with each of these antibodies. In this study, we have focused on the use of SDS for antigen retrieval using ultrathin cryosections. Herein, we show that SDS treatment can be applied to ultrathin cryosections without deleterious effects and can be used for high-resolution immunofluorescence detection of caveolin-1 and -2.
- 日本組織細胞化学会の論文
著者
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Robinson J
Department Of Physiology And Cell Biology Ohio State University
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Robinson John
Department Of Biochemistry Memorial University Of Newfoundland
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TAKIZAWA Toshihiro
Department of Molecular Anatomy, Nippon Medical School
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Takizawa Toshihiro
Department Of Anatomy Jichi Medical School
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