コマクサ培養組織のアルカロイド成分について
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概要
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Callus, adventitious embryos and regenerated plants were derived by tissue culture of the leaves of Dicentra peregrina with MS medium. The alkaloids at each stage of cultivation were analyzed by using high performance liquid chromatogarphy (HPLC). The amounts of seven alkaloids in the cultured organs were the same as in the original plant, and the total alkaloid contents in both of the dry samples were about 4%, though the alkaloid components of the callus were different from those of the adventitious embryos, regenerated and original plants, and the total alkaloid contents in the callus was also less than 1%. The ratio of the protopine content to the dicentrine content was 2 ; 1 in the adventitious embryos, and 1 : 2 in the regenerated plants. By HPLC, three new alkaloids were detected in the cultured organs, which were elucidated as (-)-trans- N-methylisocorypalmine, N-methyldicentrine and 7-hydroxy-nordicentrine.
- 1998-02-20
著者
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小西 天二
京都薬科大学
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清澤 脩
京都薬科大学
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木島 孝夫
京都薬科大学
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清沢 修
Kyoto Pharmaceutical University
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小西 一豪
京都大学薬学部
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竹村 照美
京都薬科大学
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松田 明姫
京都薬科大学
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