J0202-2-5 マイクロチャンネルを用いたヒト赤血球の形状回復に関する時定数の測定 : 膜の硬さと内部液体の粘度が及ぼす影響([J0202-2]バイオ熱・物質移動(2))

概要

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Erythrocyte is so flexible that it can pass easily through capillary with deforming. If it can't deform to pass, that causes several diseases. Thus it is very important to evaluate the dynamic deformability of erythrocyte flowing in microcirculation. So in this study, we evaluated to use a micro-channel array which has 5μm×5μm cross-section and 100μm in length on PDMS. From healthy blood, we constructed healthy erythrocyte, which is diluted by a hematocrit of 1% with PBS, hardened one, which is treated with glutaraldehyde, erythrocyte ghost. These suspensions were made to flow through the micro-channels. The motion of each erythrocyte was photographed by using a high-speed video camera. In analysis of these experiments, the shape recovery process of each erythrocyte was modeled by the Kelvin viscoelastic model. As a result of time constant of shape recovery of hardened erythrocyte is shorter than healthy. Erythrocyte ghost of time constant of shape recovery is shorter than healthy.
2010-09-04