S0201-3-3 線維芽細胞の基質からの剥離に及ぼす培養液成分と温度の影響に関する定量的評価(マイクロ・ナノバイオメカニクス:細胞生物学への接近(3)界面と接着)

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We evaluated the effects of the culture media, i.e., Dulbecco's modified Eagle medium (DMEM), Dulbecco's phosphate buffered saline (DPBS-), and DPBS+ which contained Ca and Mg ions, and the temperature on 1-hour morphological changes of fibroblasts Sire (RIKEN Cell Bank) which were seeded on the collagen coated plastic dish. We also obtained fluorescent images of single cells for DPBS+ and DPBS- after 1-hour incubation to evaluate the activity of energy production by staining mitochondria with MitoTracker Green FM. The experimental results showed that only the medium of DPBS-caused a detachment of single cells. After 1 hour the cellular area was 55% and 70% of the initial one for the temperatures of 37℃ and 25℃, respectively. The energy production was observed both for the media of DPBS+ and DPBS- even though cell bottoms were detached partially for DPBS-.
社団法人日本機械学会の論文
2009-09-12