521 栄養欠乏環境下でのコラーゲンゲル包埋線維芽細胞の共焦点および光学顕微鏡による形態観察(T10-1 マイクロ・ナノバイオテクノロジー(1):細胞の応答とその応用,大会テーマセッション,21世紀地球環境革命の機械工学:人・マイクロナノ・エネルギー・環境)

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We observed morphological changes in human skin fibroblasts, which were incubated for 6 hours, by a confocal scanning laser microscope and a light microscope under various conditions of nutrients and temperature. The cells were embedded in a collagen gel with 0.3-mm thickness. The condition of nutrition was given by using a mixture of Dulbecco's modified eagle medium and Ham's F-12 as a control and Dulbecco's phosphate buffered saline as a nutrient-free environment. The condition of temperature was given as 37℃ as a control and 25℃ which was about 10℃ lower than the control. By combining these conditions, we investigated the effect of lowering the temperature in the nutrient-free condition. The nutrient-free environment enhanced cells near the gel surface to become spherical at 37℃, while lowering the temperature to 25℃ prevented this response but did not protect cells from a reduction of their density in the gel.
社団法人日本機械学会の論文
2008-08-02