S0201-2-2 糖鎖が静置並びに流れ負荷下の血管内皮細胞の挙動に及ぼす影響(マイクロ・ナノバイオメカニクス:細胞生物学への接近(2)刺激と応答)

概要

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It has been reported that glycans play pivotal roles in biomechanical responses of cells. To reveal the roles of glycans in mechanical response of endothelial cells, we observed morphological changes of cultured mouse vascular endothelial cells treated with deoxy-D-glucose (DG), which modifies protein glycosylation. When confluent cell monolayer in static culture was treated with DG, it formed mesh-like structure in 48 hours. This change was accompanied by decrease in the total area of focal adhesions that indicates the weakening of cell-substrate adhesion. Such weakening may cause loss of ability to repair gaps between cells that may form in cell monolayer temporally. When exposed to fluid shear stress, normal cells aligned in the direction of flow while treated cells did not. These results indicate that glycans have significant effects on mechanical response of vascular endothelial cells.
2009-09-12