1005 血管平滑筋細胞の応力緩和特性に対する細胞外Ca^<2+>の影響(S03-1 細胞・生体分子のバイオメカニクス(1),S03 細胞・生体分子のバイオメカニクス)

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Stress relaxation response at the whole-cell level was studied in rat aortic smooth muscle cells (SMCs). SMCs adhering to the bottom of culture dish were held with a pair of micropipettes maintaining its in situ shape and cytoskeletal integrity. Stress relaxation test at constant strain was performed using a laboratory made micro tensile tester with pipette position feed-back control. SMCs were stretched stepwise by 30% from no-load length, and their length was kept constant for 1200s in Ca^<2+> -rich (5mM) and Ca^<2+> free condition in order to investigate the effects of extracellular Ca^<2+> on their viscoelastic properties. The stress decreased exponentially and reached a plateau in 〜300s for the majority of cells. The average stress were 〜3 times larger in the Ca^<2+> -rich condition than in the Ca^<2+> -free in all range of the stress relaxation. Although the difference in the relaxation rate was insignificant in the two groups, the stress relaxation curves were more uniform in Ca^<2+> -rich condition. These results indicate that extracellular Ca^<2+> may increase intracellular stress possibly through activating actomyosin contractility, and stabilize the viscoelastic properties of SMCs.
社団法人日本機械学会の論文
2007-09-07