740 細胞把持回転観察システムによる細胞核および細胞骨格の3次元形態観察(S08-3 細胞の構造とメカノバイオロジー(3),S08 細胞の構造とメカノバイオロジー)

概要

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A cell rotation system (CRS) has been developed to observe three dimensional (3D) morphology of cells precisely from their images obtained from various points of view. In this system, a cell held with a micropipette under a microscope is rotated around the axis of the micropipette set perpendicular to the optical axis, while the pipette tip is kept in position based on the binarized image of the pipette tip. In this study, distributing back projection (DBP) method that is one of the computerized tomography (CT) algorithms is applied to obtain clear 3D images of intracellular structure such as nucleus and cytoskeleton. The reconstructed images were clear and we could observe details of 3D structures of nucleus and actin filaments. The 3D reconstruction with CRS would become a powerfull tool for detailed observation of various intracellular structures.
社団法人日本機械学会の論文
2006-09-15