709 基板付着形状を保持した培養血管平滑筋細胞の引張特牲に対する細胞骨格の影響(S07-1 マイクロ・ナノバイオメカニクス(1),S07 マイクロ・ナノバイオメカニクス)

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Effects of actin filaments (AFs) and microtubules (MTs) on quasi-in situ tensile properties and internal tension at a whole-cell level were studied in rat aortic smooth muscle cells (SMCs) cultured on substrates. SMCs were held with a pair of micropipettes maintaining their shape on the substrate. Tensile tests up to〜10% strain were performed for 3-4 times both before and after administration of cytochalasin D or colchicine. Force-elongation curves became stable after second cycle of loading/unloading process. The cell stiffness defined as the average slope of loading curve in stable loops were decreased by〜70% after AF disruption with cytochalasin D. Treatment with colchicine, a MT disrupter, decreased cell stiffness by〜30%. The cell height significantly increased with AF disruption, while it decreased with MT disruption. These results indicate that not only AFs but also MTs play crucial roles in maintaining whole-cell stiffness of SMCs, and while AFs act as an internal tension generator, MTs may act as a tension reducer.
2006-09-15