529 大動脈平滑筋細胞内のストレスファイバの配向再現性に関する研究 : 微小管と接着斑の影響(T10-2 マイクロ・ナノバイオテクノロジー(2):細胞内部の現象を捉える,大会テーマセッション,21世紀地球環境革命の機械工学:人・マイクロナノ・エネルギー・環境)

概要

論文の詳細を見る
Stress fibers (SFs) play essential roles in various cellular functions such as cell movement, shape maintenance and cell division. In addition, changes in the mechanical environment induce reorganization of the SFs. Recently, we noticed that the SF networks reappeared following their temporal disruption with cytochalasin D looked similar to those before disruption in rat aortic smooth muscle cells (RASMs). This indicates that the cells may have a memory of SF orientation. The aim of this study was to clarify the mechanism of the memory particularly focusing on the effects of microtubules (MTs) and focal adhesions (FAs) during the reorganization process of SFs. Firstly, we preconditioned the cells with cyclic stretch to make their SFs align in a uniform direction. After cyclic stretching, we depolymerized SFs completely with cytochalasin D for 3 h, and let them repolymerize in the drug free medium for 3 h. To know the effects of MTs during the SF repolymerization, we depolymerized MTs with colchicine. FAs were observed with a surface reflective interference contrast microscopy. In the presence of MTs, repolymerized SFs appeared in random orientation with newly formed FM, while FM were not destroyed and the alignment of SFs was conserved in the absence of MTs. The conservation of FAs might serve as a memory of SF alignment.
社団法人日本機械学会の論文
2008-08-02