ずり応力惹起白血球凝集と各種薬剤による抑制

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Aggregation of leukocytes induced by shear stress may play a pivotal role in microcirculatory derangement associated with cerebral ischemia. We studied leukocyte aggregation induced by shear stress and its in vitro inhibition by various pharmacological agents. Leukocytes were separated from heparinized venous blood of healthy volunteers, and were suspended in HEPES-Tyrode's buffer containing 2% autologous platelet-poor plasma at a final concentration of 5,000/μl. A cone-plate streaming chamber was applied to determine leukocyte aggregation by inducing low shear stress (18 dynes/cm^2) and high shear stress (108 dynes/cm^2). Maximal light transmittance rates and percent reduction in leukocyte counts were measured as parameters of leukocyte aggregation. Leukocyte aggregation was found to be induced by shear stresses, which was more pronounced at low than high shear stress. Granulocytes were involved more closely in leukocyte aggregation than lymphocytes. Prostaglandins E_1 and I_2 did not affect shear-induced leukocyte aggregation (SILA). In contrast, SILA was inhibited by EGTA, as well as by anti-CD 18 and anti-CD 50 antibodies. These results indicate that SILA plays an important role in low shear circumstances such as the venous system and arterial blood stasis, and also indicate that SILA requires extracellular calcium and LFA-1/ICAM-3 cell adhesion mechanism but does not require adenylate cyclase-cyclic AMP pathway for intracellular signal transduction.
東京女子医科大学の論文
1999-08-25

ずり応力惹起白血球凝集と各種薬剤による抑制

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