Effect of moderate salinity stress treatment on the stimulation of proline uptake and growth in Escherichia coli CSH4 and its mutants under high salinity(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
Activity of proline uptake in Escherichia coli CSH4 was inhibited in the presence of 1M NaCl, while it was recovered if the cells were incubated at 30℃ for 1h in a moderate salinity stress (MSS) solution which consists of Davis minimal medium with 5mM proline and 0.5M NaCl. Then, an attempt was made to examine whether MSS treatment is also effective on the activity restoration of proline uptake and growth under high salinity for E. coli CSH4 mutants with different combinations of proP, putA, putP, and proU which are related to the transport and metabolization of proline. After MSS treatment, proline uptake was vigorously occurred for the mutants with proline transporter gene proP but not for its deficient ones. For the expression of proline uptake activities of these mutant strains after MSS treatment, PO^<3->_4 in MSS solution is more important than K^+. No growth of strain CSH4 and its mutants without MSS treatment was observed, when cultured in high osmotic medium G (0.8M NaCl) consisting of 1mM glycine betaine and Davis minimal medium without potassium phosphate supplemented. After MSS treatment, however, mutant strains lacking proP showed sufficient growth in medium G. Cell growth of proP^+ strains was recognized if MSS treatment was performed in the absence of proline. In conclusion, growth of mutant strains under high-salinity medium G depended on their amount of proline accumulated during MSS treatment, in which K^+ and PO^<3->_4 might play a key role to guarantee their sufficient growth.
著者
-
Ishida Akio
Department Of Cardiovascular Medicine Nephrology And Neurology
-
Ishida Akio
Department Of Biological Science Graduate School Of Science And Technology Kumamoto University
-
Nagata Shinichi
Environmental Biochemistry Group, Research Center for Inland Seas, Kobe University
-
Zhang Hongyan
Kobe Univ. Kobe Jpn
-
Nagata Shinichi
Environmental Biochemistry Division Research Center For Inland Seas Kobe University
-
Wang Yaoqiang
Environmental Biochemistry Division, Research Center for Inland Seas, Kobe University
-
Zhang Hongyan
Environmental Biochemistry Division, Research Center for Inland Seas, Kobe University
-
Sasaki Hideaki
College of Science and Engineering, Iwaki Meisei University
-
Oshima Akinobu
Department of Biological Science, Faculty of Life and Environmental Science, Shimane University
-
Wang Yaoqiang
Environmental Biochemistry Division Research Center For Inland Seas Kobe University
-
Oshima Akinobu
Department Of Biological Science Faculty Of Life And Environmental Science Shimane University
-
Sasaki Hideaki
College Of Science And Engineering Iwaki Meisei University
関連論文
- PJ-131 Cardiometabolic Effects Induced by Lower and Extremely Lower Doses of Diuretics in Uncontrolled Hypertension Treated with Angiotensin II Receptor Blockers(Hypertension, clinical(07)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of
- Autologous Peripheral Blood Mononuclear Cell Implantation for Patients With Peripheral Arterial Disease Improves Limb Ischemia
- Autologous Peripheral Blood Mononuclear Cells Implantation for Patients with Peripheral Artery Disease Improves Limb Ischemia(Peripheral Circulation/Vascular Disease 3 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Severe Abdominal Pain Associated with Allergic Reaction to Nafamostat Mesilate in a Chronic Hemodialysis Patient
- Analyses of Factors to Affect the Bioassay System Using Luminescent Bacterium Vibrio fischeri
- Induction of Salt Tolerance in Bacillus subtilis IFO 3025(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Effect of Compatible Solutes on the Respiratory Activity and Growth of Escherichia coli K-12 under NaCl Stress(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Supplementation Effect of Ectoine on Thermostability of Phytase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Supplementation Effects of Hydroxyectoine on Proline Uptake of Downshocked Brevibacterium sp. JCM 6894(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Inhibitory Activities of Surface Associated Bacteria Isolated from the Marine Sponge Pseudoceratina purpurea
- Antibacterial activities of marine epibiotic bacteria isolated from brown algae of Japan
- Effect of moderate salinity stress treatment on the stimulation of proline uptake and growth in Escherichia coli CSH4 and its mutants under high salinity(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- PE-213 Diabetes and Chronic Kidney Disease Contribute to Inadequate Blood Pressure Control in Hypertension Treated with Angiotensin Receptor Blocker/Diuretics(PE036,Hypertension, Clinical 2 (H),Poster Session (English),The 73rd Annual Scientific Meeting o
- Ideal Magnetohydrodynamic Stability of Static Field Reversed Configurations : Fluids, Plasmas, and Electric Discharges
- Applicability of Luminescent Assay Using Fresh Cells of Vibrio fischeri for Toxicity Evaluation
- Remarkable Synergistic Effects in Antifouling Chemicals against Vibrio fischeri in a Bioluminescent Assay
- Abiotic degradation of triphenylborane pyridine (TPBP) antifouling agent in water
- Effect of Proline and K^+ on the Stimulation of Cellular Activities in Escherichia coli K-12 under High Salinity
- Colony formations in a halotolerant Brevibacterium sp. JCM 6894 on solid medium with different pH values
- Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock
- Transcription analysis of α_2-macroglobulin in bluegill Lepomis macrochirus
- Effect of moderate osmotic treatment on the adaptation of Escherichia coli K-12 and its proline transporter mutants to hyperosmotic conditions
- Beneficial Effects of Pioglitazone on Left Ventricular Hypertrophy in Genetically Hypertensive Rats
- PJ-277 Expression of NAD(P)H Components in the Two Types of Endothelial Progenitor Cells(Regeneration (angionenesis/myocardial regeneration)-8 (M) PJ47,Poster Session (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation So
- Homocysteine Stimulates Matrix Metalloproteinase Activity in Human Vascular Smooth Muscle Cells
- ADVANCED FUELS IN A FIELD-REVERSED CONFIGURATION
- Formation of Hydrogen Peroxide by NAD(P)H Oxidation with Isolated Cell Wall-Associated Peroxidase from Cultured Liverwort Cells, Marchantia polymorpha L.
- The Baker's Mapping of a Quantum-Mechanical Path
- Isolation and Characterization of Salt-Sensitive Mutants of the Moderately Halophilic Bacterium Salinivibrio costicola subsp. yaniae
- Effect of Duration of Osmotic Downshock and Coexisting Glutamate on Survival and Uptake of Ectoine in Halotolerant Brevibacterium sp. JCM 6894(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Efficient Utilization of Ectoine by Halophilic Brevibacterium Species and Escherichia coli Subjected to Osmotic Downshock(Microbial Physiology and Biotechnology)
- On the Physical Picture for the Anomalous Propagation of an Ordinary Wave in a Magnetoplasma
- Stability of Field-Reversed Ion Rings in a Background Plasma
- Hydrogen Peroxide Induced Synthesis of Catalase in Bacillus Subtilis
- Radial Motion of a Field-Reversed Ion Layer
- Effect of Glucose on the H_2O_2-Induced Synthesis of Peroxidase in Escherichia coli
- On the Physical Picture for the Anomalous Propagation of an Ordinary Wave in a Magnetoplasma
- Anomalous Penetration of an Ordinary Wave into a Magnetoplasma Slab
- Growth Patterns and Properties of Escherichia coli Grown in Salt-Hypertonic Medium
- Effect of Glucose on the Development of Catalase Activity in Escherichia coil.
- Ideal-Magnetohydrodynamic-Stable Tilting in Field-Reversed Configurations
- Autolysis in Exponential-phase Cells of Escherichia coil
- Degradation of the Seaweed Wakame (Undaria pinnatifida) by a Composting Process with the Inoculation of Bacillus sp. HR6
- Role of Hydrogen Peroxide and Glucose on the Increase of Catalase Level in Bacillus cereus and Bacillus megaterium
- Tilt Stability of Field-Reversed Configurations
- Isolation and Characterization of Salt-Sensitive Mutants of the Moderately Halophilic Bacterium Salinivibrio costicola subsp. yaniae
- Production of the osmolyte, ectoine, from an alkaliphilic Bacillus U-21
- Low Frequency Instability in a Mirror Field
- Kink Stability of Long Ion Layers
- Effect of Acetyl-phosphate and Pyridoxalphosphate on the Induced Synthesis of Peroxidase in Pseudomonas aeruginosa
- Growth Inhibition by D-Mannosamine and Its Reversal by Glucose and Mannose in Bacillus subtilis
- Functional expression of an animal type-Na+-ATPase gene from a marine red seaweed Porphyra yezoensis increases salinity tolerance in rice plants
- Accumulation of Radioactive Cesium Released from Fukushima Daiichi Nuclear Power Plant in Terrestrial Cyanobacteria Nostoc commune