Effect of Duration of Osmotic Downshock and Coexisting Glutamate on Survival and Uptake of Ectoine in Halotolerant Brevibacterium sp. JCM 6894(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)

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Halotolerant Brevibacterium sp. JCM 6894 that was subjected to an osmotic downshock (0.7M NaCl to 0M) was examined for its survival and uptake of ectoine in the presence of ectoine and/or carbon sources. In the presence of ectoine alone, the rates of ectoine uptake by the 1h-down-shocked cells were low and high in the absence and presence of 0.7M NaCl, respectively, which were in parallel with the rates of cell growth. The presence of glutamate or amino acids together with ectoine exerted a stimulative effect on the survival of downshocked cells. The incubation time of the cells subjected to osmotic downshock strongly affected ectoine uptake as well as the cell growth of this strain, suggesting that the transporter of ectoine in the strain JCM 6894 was stimulated during the osmotic downshock for about 1h. Different downshock strengths had marked effects on the rate of ectoine uptake when the downshocked cells were incubated in the presence of NaCl.
社団法人日本生物工学会の論文
2006-01-25