Supplementation Effect of Ectoine on Thermostability of Phytase(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
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概要
- 論文の詳細を見る
In this study, we elucidated the supplementation effect of compatible solutes on the thermostability of phytase, designated as PHYA II, which was encoded by the phytase gene phyA I (GeneBank AY013315) from Aspergillus ficuum As3.324 and expressed in Pichia pastoris GS115. When PHYA II in acetate buffer was heated at 90℃ for 15min, more than 80% of the residual activity was retained by adding the cyclic amino acid ectoine, a representative compatible solute. Furthermore, the presence of ectoine led to an increase in the relative hydrolytic rate of sodium phytate by 15.7% with heating at 80℃ for 15min. Among the compatible solutes examined, ectoine was confirmed to be the most efficient thermoprotectant for PHYA II.
- 社団法人日本生物工学会の論文
- 2006-12-25
著者
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Nagata Shinichi
Environmental Biochemistry Group, Research Center for Inland Seas, Kobe University
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Zhu Daochen
Kobe Univ. Kobe Jpn
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Nagata Shinichi
Environmental Biochemistry Division Research Center For Inland Seas Kobe University
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Nagata Shinichi
Kobe Univ. Kobe Jpn
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Zhang Linghua
College of Environmental Science and Engineering, Dalian Maritime University
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Wang Yue
College of Environmental Science and Engineering, Dalian Maritime University
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Zhang Chunyu
College of Environmental Science and Engineering, Dalian Maritime University
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Wang Yunji
College of Bio & Food Technology, Dalian Institute of Light Industry
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Zhu Daochen
Environmental Biochemistry Division, Research Center for Inland Seas, Kobe University
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Wang Chenxiang
Environmental Biochemistry Division, Research Center for Inland Seas, Kobe University
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Zhang Chunyu
College Of Environmental Science And Engineering Dalian Maritime University
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Zhang Linghua
College Of Environmental Science And Engineering Dalian Maritime University:(present Address) Colleg
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Wang Chenxiang
Environmental Biochemistry Group Research Center For Inland Seas Kobe University
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Zhu Daochen
Environmental Biochemistry Division Research Center For Inland Seas Kobe University
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Wang Yue
College Of Environmental Science And Engineering Dalian Maritime University:college Of Bio & Foo
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Wang Chehxiang
Environmental Biochemistry Group, Research Center for Inland Seas, Kobe University
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