Efficient Utilization of Ectoine by Halophilic Brevibacterium Species and Escherichia coli Subjected to Osmotic Downshock(Microbial Physiology and Biotechnology)
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概要
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Halophilic and non-halophilic bacteria subjected to osmotic downshock, from 0.7 M NaCl to deionized water, were examined for their survival, with the uptake and utilization of the cyclic amino acid ectoine, one of the representative compatible solutes, being taken into account. The uptake of ectoine added externally and survival of the cells were monitored as a function of incubation time in the presence and absence of NaCl. The halophilic Brevibacterium sp. JCM 6894 and B. epidermidis JCM 2593 actively accumulated ectoine regardless of the presence of NaCl, which led to cell survival. Brevibacterium casei JCM 2594 belonging to the same Brevibacterium species, however, revealed Na^+-dependence of its uptake activity of ectoine. Non-halophilic Escherichia coli K-12 did not accumulate ectoine, and thereby this strain failed to survive irrespective of whether NaCl was present. The physiological meanings of the downshock procedure are discussed in connection with the uptake and the subsequent utilization of ectoine.
- 社団法人日本生物工学会の論文
- 2005-01-25
著者
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Nagata Shinichi
Environmental Biochemistry Group, Research Center for Inland Seas, Kobe University
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Nagata Shinichi
Environmental Biochemistry Division Research Center For Inland Seas Kobe University
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Nagata Shinichi
Environmental Biochemistry Group Research Center For Inland Seas Kobe University
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Wang Chenxiang
Environmental Biochemistry Division, Research Center for Inland Seas, Kobe University
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Wang Chenxiang
Environmental Biochemistry Group Research Center For Inland Seas Kobe University
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