***におけるアルデヒド脱水素酵素遺伝子分布と体質判定法の検討

概要

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Genetic and epidemiological studies on alcohol tolerance frequencies were conducted by analyzing genetic materials from 149 healthy female students. Aldehyde dehydrogenase 2 (ALDH2) gene was characterized by the combination of polymerase chain reaction and agarose gel electrophoresis of respective DNA specimen. Nail clippings were found to be relatively easy to collect and extract DNA from volunteers especially when they were restricted to female students. Plucking hair roots was not successful, since it caused sometimes pains on the volunteers. Among 149 individuals tested, 65.6% were the typical or normal homozygote, 32.2% were the heterozygote, and 2.0% were the atypical mutant homozygote. The reliability of the scale ratings using ALDH2 genotype analysis was assessed and confirmed by ethanol patch test and other psychiatrist's evaluation methods, such as TAST (Tokyo-University ALDH2-Phenotype Screening Test). However, the genotype analysis is not directly associated with AAIS (Adolescent Alcohol Involvement Scale). Accordingly, careful case-control study must be undertaken on genotype analysis and it must be accompanied by several combination of epidemiological studies.
日本食品化学学会の論文
2005-12-30