スジエビPalaemon Paucidensのセレブロシド

概要

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Cerebroside (monoglycosylceramide) was purified from the fresh-water shrimp Palaemon paucidens (Phylum, Arthropoda; Class, Crustacea). An alkali-stable lipid fraction (6.6g) prepared from the chloroform-methanol extracts of the shrimp whole tissues was successively fractionated on ion-exchange (QAE-Sephadex) and silicic acid (Iatrobeads) column chromatography. The purified cerebroside (6.5mg) was characterized to be a glucocerebroside by gas-liquid chromatography (GC), gas chromatograph-mass spectrometry (GC-MS), matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) and 1H-nuclear magnetic resonance spectroscopy (NMR). The ceramide moiety was mainly composed of saturated C20, C22 and C24 acids as the fatty acids, and saturated and mono-unsaturated bases (C14～C16) as the sphingoids.
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