Expression and Significance of Angiopoietin-1, 2 and Tie-2 Receptor in Human Extrahepatic Bile Duct Carcinoma: Correlation with Clinicopathological Factors
スポンサーリンク
概要
- 論文の詳細を見る
Extrahepatic bile duct cancer is a high mortal malignancy. Angiopoietin (Ang) and its receptor Tie, which are known to contribute to angiogenesis, have recently been reported to participate in the proliferation and differentiation of malignant tumor cells. The aim of this study is to investigate the expression and the significance of Ang-1, 2 and Tie-2 in extrahepatic bile duct carcinoma cells. We used immunohistochemistry to study 119 cases of surgically resected human extrahepatic bile duct carcinoma, and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) to confirm the expression of Ang-1, 2 and Tie-2 mRNA. Among these 119 cases, 52 (43.7%), 50 (42.0%) and 89 (74.8%) cases showed positive staining for Ang-1, 2 and Tie-2, respectively, in bile duct carcinoma cells. In 38 cases of normal mucosa, 6 (15.8%), 10 (26.3%) and 9 (23.7%) cases were positive for Ang-1, 2 and Tie-2, respectively. The positivity for Ang-1 and Tie-2 in normal mucosa was significantly different from all carcinomas (p<0.01 and p<0.001, respectively). We found no significant correlation between Ang-1 and Ang-2 expression and other clinicopathological factors such as histological differentiation, grade of tumor invasion or survival rate after surgery. In contrast, Tie-2 expression correlated significantly with degree of desmoplasia, cancer stage and survival of patients. RT-PCR analyses of five surgically resected tumor samples and three human bile duct cancer cell lines all showed positive expression of Ang-1, 2 and Tie-2 mRNAs. High expressions of Ang-1, 2 and Tie-2 in human extrahepatic bile duct carcinoma cells suggested that Ang-Tie system may be involved in the progression of human bile duct cancer.
- 長崎大学の論文
著者
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Kuroki Tamotsu
Department Of Transplantation And Digestive Surgery Nagasaki University Graduate School Of Biomedica
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Kuroki Tamotsu
Department Of Surgery Goto Central Hospital
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HAYASHI Tomayoshi
Department of Pathology, Nagasaki University Hospital of Medicine and Dentistry
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Mihara Yumi
Department Of Tumor And Diagnostic Pathology Nagasaki University Graduate School Of Biomedical Scien
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ITO MASAHIRO
Department of Pediatrics, School of Medicine, Mie University
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Ito Masahiro
Department Of Pathology National Nagasaki Medical Center
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NANASHIMA ATSUSHI
Department of Translational Medical Sciences, Division of Surgical Oncology, Nagaski University Grad
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Onizuka Shinya
Department of Surgery, NHO Nagasaki Medical Center
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Sanefuji Hayato.
Department of Pathology, Kitakyushu General Hospital
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Nakayama Toshiyuki
Department Of Hematology Kouseiren Tsurumi Hospital
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Hayashi Tomayoshi
Department Of Pathology Nagasaki University Hospital Of Medicine And Dentistry
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Onizuka Shinya
Department Of Surgery Nho Nagasaki Medical Center
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Onizuka Shinya
Department Of Surgery National Nagasaki Medical Center
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Hayashi Tomayoshi
Department Of Cardiovascular Surgery And Pathology Nagasaki University School Of Medicine
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Sanefuji Hayato.
Department Of Pathology Kitakyushu General Hospital
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Nanashima Atsushi
Department Of Surgical Oncology Nagasaki University Graduate School Of Biomedical Sciences
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Ito Masahiro
Department Of Gastroenterological Surgery Fujita Health University
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Ito Masahiro
Department Of Applied Chemistry Faculty Of Engineering Osaka University
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Ito Masahiro
Department Of Pathology Nho Nagasaki Medical Center
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Mihara Yumi
Department Of Pathology National Nagasaki Medical Center
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Naruke Yuki
Department Of Pathology National Nagasaki Medical Center
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Onizuka Shinya
Department of Pathology Atomic Disease Institute, Nagasaki University School of Medicine
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