Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells

スポンサーリンク

概要

• 論文の詳細を見る

• The definitive version is available at www.blackwell-synergy.comMicro RNAs (miRNAs) are non-coding small RNAs and constitute a novel class of negative gene regulators that are found in both plants and animals. Several miRNAs play crucial roles in cancer cell growth. To identify miRNAs specifically deregulated in anaplastic thyroid cancer (ATC) cells, we performed a comprehensive analysis of miRNA expressions in ARO cells and primary thyrocytes using miRNA microarrays. MiRNAs in a miR-17-92 cluster were overexpressed in ARO cells. We confirmed the overexpression of those miRNAs by Northern blot analysis in ARO and FRO cells. In 3 of 6 clinical ATC samples, miR-17-3p and miR-17-5p were robustly overexpressed in cancer lesions compared to adjacent normal tissue. To investigate the functional role of these miRNAs in ATC cells, ARO and FRO cells were transfected with miRNA inhibitors, antisense oligonucleotides containing locked nucleic acids. Suppression of miR-17-3p caused complete growth arrest, presumably due to caspase activation resulting in apoptosis. MiR-17-5p or miR-19a inhibitor also induced strong growth reduction, but only miR-17-5p inhibitor led to cellular senescence. On the other hand, miR-18a inhibitor only moderately attenuated the cell growth. Thus, we have clarified functional differences among the members of the cluster in ATC cells. In conclusion, these findings suggest that the miR-17-92 cluster plays an important role in certain types of ATCs and could be a novel target for ATC treatment.

• Blackwell Publishingの論文
• 2008-06-10

著者

• NAKASHIMA Masahiro

  Tissue and Histopathology Section, Division of Scientific Data Registry, Nagasaki University Graduat

• Takakura Shu

  Department Of Molecular Medicine Nagasaki University Graduate School Of Biomedical Sciences

• Mitsutake Norisato

  Department Of Molecular Medicine Atomic Bomb Disease Institute Nagasaki University Graduate School O

• Mitsutake Norisato

  長崎大学大学院医歯薬学総合研究科附属原爆後障害医療研究施設 分子医療部門

• Saenko V

  Department Of International Health And Radiation Research Atomic Bomb Disease Institute Nagasaki Uni

• Saenko Vladimir

  長崎大学大学院医歯薬学総合研究科附属原爆後障害医療研究施設 分子医療部門分子診断研究分野

• Saenko Vladimir

  長崎大学 医歯薬学総合研究科展開医療科学講座形成再建外科学

• Yamashita S

  Department Of Molecular Medicine Atomic Bomb Disease Institute Nagasaki University Graduate School O

• Yamashita S

  Department Of Molecular Medicine Nagasaki University School Of Medicine

• Yamashita S

  Radiation And Environmental Health Department Of Protection Of The Human Environment World Health Or

• NAMBA Hiroyuki

  Department of Molecular Medicine, Nagasaki University Graduate School of Biomedical Sciences

• SAENKO Vladimir

  Department of International Health and Radiation Research, Atomic Bomb Disease Institute, Nagasaki U

• ROGOUNOVITCH Tatiana

  Department of Molecular Medicine, Nagasaki University Graduate School of Biomedical Sciences

• NAKAZAWA Yuka

  Department of Molecular Medicine, Nagasaki University Graduate School of Biomedical Sciences

• HAYASHI Tomayoshi

  Department of Pathology, Nagasaki University Hospital of Medicine and Dentistry

• OHTSURU Akira

  Takashi Nagai Memorial International Hibakusha Medical Center, Nagasaki University Hospital of Medic

• YAMASHITA Shunichi

  Department of Molecular Medicine, Nagasaki University Graduate School of Biomedical Sciences

• Saenko Vladimir

  Department Of International Health And Radiation Research Atomic Bomb Disease Institute Nagasaki Uni

• Namba Hiroyuki

  Department Of Molecular Medicine Nagasaki University Graduate School Of Biomedical Sciences

• Nakazawa Yuka

  Department Of Molecular Medicine Atomic Bomb Disease Institute Nagasaki University Graduate School O

• Ohtsuru A

  Takashi Nagai Memorial International Hibakusha Medical Center Nagasaki University Hospital Of Medici

• Ohtsuru Akira

  長崎大学大学院医歯薬学総合研究科附属原爆後障害医療研究施設 分子生物学

• Ohtsuru Akira

  長崎大学大学院医歯薬学総合研究科附属原爆後障害医療研究施設 分子医療部門

• Ohtsuru Akira

  Takashi Nagai Memorial International Hibakusha Medical Center

• Yamashita Shunichi

  Department Of Molecular Medicine Atomic Bomb Disease Institute Nagasaki University Graduate School O

• HAYASHI Tomayoshi

  Nagasaki University Hospital

• Nakashima Masahiro

  Tissue And Histopathology Section Division Of Scientific Data Registry Atomic Bomb Disease Institute

• Shibata Yoshisada

  長崎大学医学部附属原爆後障害医療研究施設 放射線疫学 研究分野

• Shibata Yoshisada

  Atomic Bomb Disease Institute Nagasaki Univ. Graduate Sch. Biomedical Sciences

• Nakashima Masahiko

  Tissue And Histopathology Section Division Of Scientific Data Registry Atomic Bomb Disease Institute

• Nakashima Masahiro

  長崎大医歯薬学総合研究科原研 病態分子解析

• Nakashima Masahiro

  Tissue And Histopathology Section Division Of Scientific Data Registry Nagasaki University Graduate

• Namba Hiroyuki

  Department Of Cell Physoloy Atomin Disease Institute Nagasaki University School Of Medicint

• Yamashita Shunichi

  Dep. Of Molecular Medicine Nagasaki Univ. Graduate School Of Biomedical Sciences

• Hayashi Tomayoshi

  Department Of Cardiovascular Surgery And Pathology Nagasaki University School Of Medicine

• Hayashi Tomayoshi

  Deaprtment Of Pathology Nagasaki University Hospital

• Ohtsuru A

  Department Of Nature Medicine Atomic Bomb Disease Institute Nagasaki University School Of Medicine

• Shibata Yoshisada

  Department Of Molecular Medicine Atomic Bomb Disease Institute Nagasaki University Graduate School O

• Saenko Vladimir

  Department Of International Health And Radiation Research Atomic Bomb Disease Inst. Nagasaki Univ. S

• Rogounovitch Tatiana

  Department Of Molecular Medicine Atomic Bomb Disease Institute Nagasaki University Graduate School O

• Rogounovitch Tatiana

  Department Of Nature Medicine Atomic Bomb Disease Inst. Nagasaki Univ. Sch. Med.

• Yamashita Shunichi

  Department Of Cell Physiology Atomic Disease Institute Nagasaki University School Of Medicine

• Hara Takeshi

  Department Of Cell Physiology Atomic Disease Institute Nagasaki University School Of Medicine

• Shibata Yosisada

  Rerf

• Saenko Vladimir

  Department Of Health Risk Control Nagasaki University Graduate School Of Biomedical Sciences

関連論文

• Alteration of p53-binding protein 1 expression during skin carcinogenesis: association with genomic instability.
• Oncogenic role of miR-17-92 cluster in anaplastic thyroid cancer cells
• No Evidence of ARAF, CRAF and MET Mutations in BRAF^ Negative Human Papillary Thyroid Carcinoma
• Immunohistochemical analysis of connexin43 expression in infertile human testes.
• No.37 Molecular epidemiology study of radiation-induced thyroid cancer as a main research project of Representative office of Nagasaki University in Belarus
• Hepatitis B Related Polyarteritis Nodosa Presenting Necrotizing Vasculitis in the Hepatobiliary System Successfully Treated with Lamivudine, Plasmapheresis and Glucocorticoid
• Ukraine Urinary Iodine Levels ; 20 years after the Chernobyl Accident
• Plasma Concentrations of Adrenomedullin and Ghrelin in Hemodialysis Patients with Sustained and Episodic Hypotension
• Steam Leak Accident at Nuclear Power Plant in Japan
• Thyroid Blockade during a Radiation Emergency in Iodine-rich Areas : Effect of a Stable-iodine Dosage
• Benign Thyroid Diseases among Chernobyl Liquidators
• Urinary lodine Kinetics after Oral Loading of Potassium lodine
• No Evidence of Radiation Risk for Thyroid Gland among Schoolchildren around Semipalatinsk Nuclear Testing Site
• Lung fibrosis 10 years after cessation of bleomycin therapy.
• Primary High-Grade Myofibroblastic Sarcoma Arising From the Pericardium
• Acute Radiation Effects of the Nagasaki Atomic Bomb by Distance and Shielding Condition of Exposure
• Analysis of Factors Related to Mental Health of Atomic Bomb Survivors
• A Mail Survey of Mental Health Status in Atomic Bomb Survivors in Nagasaki
• Geographical Study on the Frequency of Epilation among Atomic Bomb Survivors in Nagasaki
• Correlation between Acute Radiation Injuries and Hematological Parameters in Atomic Bomb Survivors
• Mental health conditions among atomic bomb survivors in Nagasaki
• Acute Radiation Injuries and Hematological Parameters Measured 25 Years After the Bombing in AtomicBomb Survivors
• Accuracy of Atomic Bomb Survivors' Memory for Acute Symptoms
• Correlation between lifestyle and mortality in atomic bomb survivors
• Intraductal papillary neoplasm of the bile duct extending superficially from the intrahepatic to extrahepatic bile duct
• Risk of Development of Abdominal Aortic Aneurysm and Dissection of Thoracic Aorta in a Postpartum Womanwith Marfans Syndrome
• Mutation Analysis of RAP1 Gene in Papillary Thyroid Carcinomas
• Elevated Levels of Tenascin-C in Patients with Cryptogenic Organizing Pneumonia
• Nonspecific Interstitial Pneumonia with Poor Prognosis Associated with Amyopathic Dermatomyositis
• 3 Geographic Effects on Cancer Mortality in Nagasaki A-Bomb Survivors(Atomic bomb effects, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Segmental Jumping Translocation of Ret Oncogene in Radiation-associated Thyroid Cancer
• Mucosa-Associated Lymphoid Tissue Lymphoma of the Thymus Associated with Sjogren's Syndrome : Report of a Case
• Contrast-Enhanced Dynamic Magnetic Resonance Imaging of Primary Pulmonary Leiomyosarcoma
• DHMEQ, a novel NF-kappaB inhibitor, suppresses growth and type I collagen accumulation in keloid fibroblasts
• A Novel Role for Thyroid Hormone Receptor Beta in Cellular Radiosensitivity
• Expression and Significance of Angiopoietin-1, 2 and Tie-2 Receptor in Human Extrahepatic Bile Duct Carcinoma: Correlation with Clinicopathological Factors
• Urinary Iodine Levels and Thyroid Diseases in Children; Comparison between Nagasaki and Chernobyl
• Establishment of Telemedicine System for International Hibakushas' Medical Care
• A Single Missense Mutation in Codon 918 of the RET Proto-Oncogene in Sporadic Medullary Thyroid Carcinomas
• Ruptured Aneurysm of the Visceral Artery : Report of Two Cases
• Peripheral T-cell lymphoma with a nodular growth pattern
• Amyloid a Protein Amyloidosis in a Patient with Plasma Cell Dyscrasia
• A case of cold agglutinin disease in the course of treatment for polymyalgia rheumatica
• Elevation of Serum KL-6 Glycoprotein or Surfactant Protein-D in Adult T-cell Leukemia with Distinct Pulmonary Complications
• Apoptosis of articular chondrocytes in rheumatoid arthritis and osteoarthritis : correlation of apoptosis with degree of cartilage destruction and expression of apoptosis-related proteins of p53 and c-myc
• cDNA Cloning and Chromosomal Mapping of Rat Smad2 and Smad4 and Their Expression in Cultured Rat Articular Chondrocytes
• Transforming Growth Factor-β Stimulates Articular Chondrocyte Cell Growth through p44/42 MAP Kinase (ERK) Activation
• Regulationof c-fos Gene lnduction and Mitogenic Effect of Transforming Growth Factor-β1 in Rat Articular Chondrocyte
• Effect of 22-Oxa-1, 25-Dihydroxyvitamin D_3 on Human Thyroid Cancer Cell Growth
• Chemosensitivity of a Recurrent Desmoid Tumor with Increased Osteopontin Expression and a Novel Frame-Shift Mutation at Codon 1564 of the APC Gene
• Histological analysis of the ligamentum flavum of patients with dialysis-related spondyloarthropathy
• 30-III-06 Estrogen receptor-associated expression of keratinocyte growth factor and its possible role in the inhibition of the induction of apoptosis in human breast cancer(THE JOINT MEETING OF THE 44TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTR
• Incidence of multiple primary cancers in Nagasaki atomic bomb survivors: association with radiation exposure.
• Correlation of donor-derived keratinocytes and severity of graft-versus-host disease (GVHD) in epidermis
• 40 Cyclin D1 Overexpression in Thyroid Tumors from the Radio-contaminated Area, and its Correlation with Pin1 and Aberrant beta-Catenin Expressions(Radiation carcinogenesis, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• A case of pleomorphic carcinoma of the lung
• Attenuated Familial Adenomatous Polyposis Associated with Advanced Rectal Cancer in a 16-Year-Old Boy : Report of a Case
• Small-Cell Carcinoma of the Gallbladder : Report of a Case
• Pediatric CT scan usage in Japan : results of a hospital survey
• Multiple Primary Cancers in Nagasaki Atomic Bomb Survivors
• Vitamin D3 Treatment for Locally Advanced Thyroid Cancer : A Case Report
• Novel tumorigenic rfp/ret fusion oncoprotein in radiation induced papillary thyroid carcinoma
• Characteristic changes in mitochondrial DNA in radiation-associated human thyroid tumors
• Expression of Wnt Signaling Pathway in Thyroid Cancers from Residents living around Semipalatinsk NuclearTest Site
• Osteogenic Action of Parathyroid Hormone-Related Peptide (1-141) in Rat ROS Cells
• Molecular Pathology of Radiation-Induced Thyroid Cancer
• Childhood Thyroid Cancer:Comparison of Japan and Belarus
• A Case of Adrenal Insufficiency Due to Acquired Hypothalamic CRH Deficiency
• Irradiated fibroblast-induced bystander effects on invasive growth of squamous cell carcinoma under cancer-stromal cell interaction
• Protection by Polaprezinc Against Radiation-Induced Apoptosis in Rat Jejunal Crypt Cells
• Sucralfate Protects Intestinal Epithelial Cells from Radiation-Induced Apoptosis in Rats
• Immunohistochemical examination of mucinous cystadenoma of the testis
• The Protective Effect of Fermented Milk Kefir on Radiation-induced Apoptosis in Colonic Crypt Cells of Rats
• Expression of Tie-2 and angiopoietin-1 and -2 in early phase of ulcer healing
• The protective effect of a fermented milk Kefir to radiation-induced apoptosis in rat colon
• Cyclin D1 overexpression in Thyroid Tumors in the Radio-contaminated area after the Chernobyl accident, and its Relations with Aberrant betacatenin and Pin1 expression
• Involvement of Wnt Pathway during Colonic Carcinogenesis Induced by Irradiation
• Segmental Mediolytic Arteriopathy Involving Celiac to Splenic and Left Renal Arteries
• SNPs in the XRCC3 and MTF-1 genes and risk of radiation-induced papillary thyroid cancer in children (第48回原子爆弾後障害研究会 特集号)
• Mitochondrial DNA and Human Thyroid Diseases
• 209 Molecular analysis of mitochondrial DNA in solid organs of irradiated rats(Physics, chemistry and DNA damage, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• 35 Arg/Pro TP53 codon 72 polymorphism in radiation-associated human thyroid tumors(Radiation carcinogenesis, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• 2 Correlation between lifestyle and mortality in Atomic Bomb Survivors(Atomic bomb effects, Abstracts of the 46th Annual Meeting of the Japan Radiation Research Society)
• Autocrine/Paracrine Involvement of Parathyroid Hormone-Related Peptide in Vascular Leiomyoma
• The suppression effect of sucralfate and soduim butyrate on radiation-induced apoptosis
• Current and Future Strategy for Breast Cancer Treatment at Nagasaki University Hospital
• The sympathetic nervous system promotes carbon tetrachloride-induced liver cirrhosis in rats by suppressing apoptosis and enhancing the growth kinetics of regenerating hepatocytes
• Involvement of Parathyroid Hormone-Related Peptide in Cell Proliferation Activity of Human Uterine Leiomyomas
• Gastric cancer associated with overexpression of parathyroid hormone-related peptide (PTHrP) and PTH/PTHrP receptor in relation to tumor progression
• Involvement of c-Fos proto-oncogene during palatal fusion and interdigital space formation in the rat
• Establishment and Characterization of a Malignant Epithelioid Hemangioendothelioma from Mouse Thyroid Tumor
• Histopatbological findings of childhood thyroid cancer in Gome, Belarus
• Usefulness of thyroid ultrasound and fine needle aspiration cytology to evaluate the incidence of thyroid diseases in children around Chemobyl
• OE-297 Is Soluble Lectin-like Oxidized Low-density Lipoprotein Receptor-1 a Good Indicator for Early Detection of Myocardial Fibrosis in Hypertrophic Cardiomyopathy?(Cardiomyopathy, basic/clinical-1, The 71st Annual Scientific Meeting of the Japanese Circ
• Radiation effect on the Epidennal Growth Factor Binding and on the EGF dependent EGFR-Tyrosine Phosphorylation
• Expression of keratinocyte growth factor and its receptor in human breast cancer : its inhibitory role in the induction of apoptosis possibly through the overexpression of Bcl-2
• II-B-15 Estrogen receptor-associted expression of keratinocyte growth factor (KGF) and its possible involvement in the regulation of cell kinetics in human breast cancer in vivo and in vitro(THE 45TH ANNUAL MEETING OF THE JAPAN SOCIETY OF HISTOCHEMISTRY A
• Cloning and Characterization of Cell Strains Derived from Human Corneal Stroma and Sclera
• Mutational and clinico-pathological analysis of papillary thyroid carcinoma in Serbia
• Surgical management of primary thyroid carcinoma arising in thyroglossal duct cyst : An experience of a single institution in Serbia

もっと見る 閉じる

スポンサーリンク