Why Do Flocculent Yeast Cells Not Flocculate in Early Growth Phase?

概要

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Cells of Saccharomyces cerevisiae IFO 2018 began to flocculate in the middle exponential phase, while they were dispersed in the early-growth phase. Cycloheximide strongly inhibited induction of floc-forming ability of the early-phase cells when the antibiotic was added to the growing culture. A definite co-flocculation occurred when the non-flocculent early-phase cells and flocculent cells were mixed. Photo-irradiation of early-phase cells in the presence of methylene blue did not weaken the co-flocculation with flocculent cells. After treatment with mercaptoethanol, early-phase cells co-flocculated with the complete cells much more strongly. The early-phase cells treated with periodate failed to co-flocculate with the complete cells. On the other hand, the complete cells deflocculated by treatment with mercaptoethanol or photo-irradiation did not co-flocculate with early-phase cells. Although flocculent cells deflocculated by periodate oxidation failed to co-flocculate with early-phase cells, mercaptoethanol treatment of the early-phase cells promoted the co-flocculation. It is suggested that early-phase cells are not able to produce surface protein component essential for self-flocculation.
香川大学の論文