Effects of Genistein on Oxidative Injury in Endothelial Cells
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概要
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The aim of this study was to test the hypothesis that genistein protects vascular endothelial cells against the pro-atherosclerotic stressor, oxidized low-density lipoprotein (ox-LDL), by inducing antioxidant enzymes and preventing apoptosis. Human umbilical cord-derived endothelial cells (ECV 304) were incubated with genistein (10-100μmol/L), the radical scavenging antioxidant vitamin E (α-tocopherol, 50μmol/L), or vehicle for 24h and then were incubated with ox-LDL for an additional 24h. Subsequently, antioxidant enzyme activities, lipid peroxidation, adhesion to monocytes, cell morphology, viability and apoptotic index were assessed. Ox-LDL decreased superoxide dismutase and glutathione peroxidase activities in endothelial cells and caused lipid peroxidation, adhesion to monocytes, morphological injury and apoptosis (p<0.05). These effects were prevented by vitamin E and dose-dependently by genistein (p<0.05). Further, this effect of genistein is associated with maintenance of antioxidant enzyme activities and inhibition of lipid peroxidation.
著者
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Wilson John
Department Of Exercise And Nutrition Sciences School Of Public Health And Health Professions Univers
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Chang Hong
Department Of Nutrition And Food Hygiene School Of Public Health Tianjin Medical University
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Huang Guowei
Tianjin Key Laboratory Of Food Nutrition And Safety College Of Food Engineering And Biotechnology Ti
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LIU Yinghua
Department of Nutrition, Chinese PLA General Hospital
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Cao Xiaohong
Tianjin Key Laboratory Of Food Nutrition And Safety College Of Food Engineering And Biotechnology Ti
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ZHANG Xumei
Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University
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REN Dalin
Department of Nutrition and Food Hygiene, School of Public Health, Tianjin Medical University
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Chang Hong
Department Of Hematology West China Hospital Sichuan University
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Ren Dalin
Department Of Nutrition And Food Hygiene School Of Public Health Tianjin Medical University
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Zhang Xumei
Department Of Nutrition And Food Hygiene School Of Public Health Tianjin Medical University
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Liu Yinghua
Department Of Nutrition General Hospital Of Chinese Pla
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Liu Yinghua
Department Of Nutrition Chinese Pla General Hospital
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