AFLP (Amplified Fragment Length Polymorphism:増幅断片長多型)解析による3倍性ギンブナ(Carassius auratus langsdorfi)に特徴的なゲノムマーカーの探索

概要

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The Japanese silver crucian carp (so-called ginbuna, Carassius auratus langsdrofi) has two reproduction systems; one is a sexual reproduction practiced by diploid individuals and the other is gynogenetic reproduction by triploid individuals. In this study, AFLP (Amplified Fragment Length Polymorphism) analysis was carried out to isolate and characterize genomic DNA markers for triploid ginbuna, as a step toward revealing the genomic makeup and origin of triploid ginbuna. Two valuable DNA markers were isolated from 14 fragments cloned from 3 AFLP fragments in the triploid ginbuna genome. Specific PCR analyses to these DNA markers and their sequencing analyses allowed us to divide the triploid ginbuna DNA into 6 genomic types (type A〜F). The PCR products from many triploid ginbuna individuals comprising type A, C, D and E were shared with those from diploid ginbuna and those from the continental silver crucian carp, C. a. gibelio. The triploid ginbuna of type B appears to be derived from the gibelio crucian carp. These data provide additional evidence for the multiple origins of the triploid ginbuna and the genomic contribution made by the diploid ginbuna and the gibelio crucian carp to the triploid hybrid ginbuna.