Identification and Sterilization of Epiphytic Bacterial Flora near Hydrangea Shoot Apical Meristems
スポンサーリンク
概要
- 論文の詳細を見る
In some plant species, bacterial contamination frequently occurs in in vitro culture. Also, in hydrangea (Hydrangea spp.), microbial contamination frequently occurs when their explants are cultured in vitro. We identified bacterial flora near hydrangea shoot apical meristems (SAMs) by analyzing their 16S ribosomal DNA (16S rDNA) sequences. Sequences of 16S rDNA fragments amplified from bacteria isolated from SAMs of 8 hydrangea cultivars were identical to those of 12 bacterial species. Because 1 to 9 bacterial species were detected only once in each cultivar, and we focused on bacteria that can colonize on Nutrient Broth medium, in this reserch we could not cover all bacterial species existing near SAMs. The appropriate chlorine concentration for sterilizing SAMs was decided using 'Miss Hepburn'. When shoot tips with stripped SAMs were dipped in chlorine solution with 0.0005%-0.5% available chlorine concentration for 30min, no bacterial colony was observed in the treatment with 0.05% chlorine. From this result, direct exposure of the stripped meristem to chlorine solution is a suitable method for sterilizing hydrangea shoot tip culture explants, and the bacteria causing in vitro contamination in hydrangea shoot-tip culture are not endophytic but epiphytic. A successful method for in vitro culture of hydrangea was established using 8 hydrangea cultivars in Exp. 3 and in 7 cultivars, we could gain 5 to 19 viable plants from 20 cultured explants without bacterial contamination by sterilizing shoot tips for 30min with a sterilization solution of 0.05% available chlorine concentration after removal of all but two pairs of leaf primordia. No viable explant was gained from 'Flambeau', so the chlorine concentration must be reconsidered to sterilize such cultivars that have chlorine-sensitive meristems. One hundred and thirty days after culture initiation, no contamination was observed and cultured explants grew sufficiently to be transplanted out of the bottle. Surface sterilization of the SAM can be applied for many plants in which bacterial contamination poses a big problem in shoot-tip culture.
- 園芸学会の論文
著者
-
TANAKA Chihiro
Graduate School of Agriculture, Kyoto University
-
HOSOKAWA Munetaka
Graduate School of Agriculture, Kyoto University
-
Yazawa Susumu
Graduate School Of Agriculture Kyoto University
-
Tanaka Chihiro
Graduate School Of Agriculture Kyoto University
-
Yazawa Susumu
Laboratory Of Vegetable And Ornamental Horticulture Department Of Agronomy And Horticultural Science
-
Kitamura Yoshikuni
Graduate School of Agriculture, Kyoto University
-
Kitamura Yoshikuni
Graduate School Of Agriculture Kyoto University
-
Hosokawa Munetaka
Laboratory Of Vegetable And Ornamental Horticulture Department Of Agronomy And Horticultural Science
-
Hosokawa Munetaka
Graduate School Of Agriculture Kyoto University
関連論文
- Association of Genetic Polymorphisms of Endothelin-Converting Enzyme-1 Gene with Hypertension in a Japanese Population and Rare Missense Mutation in Preproendothelin-1 in Japanese Hypertensives
- Association of Genetic Polymorphisms of Sodium-Calcium Exchanger 1 Gene, NCX1, with Hypertension in a Japanese General Population
- Identification of 108 SNPs in TSC, WNK1, and WNK4 and their association with hypertension in a Japanese general population
- Association of PLA2G7 polymorphisms with carotid atherosclerosis in hypertensive Japanese
- Single Nucleotide Polymorphisms in the Interleukin-6 Gene Associated with Blood Pressure and Atherosclerosis in a Japanese General Population
- Characterization and genetic analysis of laboratory mutants of Cochliobolus heterostrophus resistant to dicarboximide and phenylpyrrole fungicides
- ITS rDNA variation of the Coprinopsis phlyctidospora (syn. : Coprinus phlyctidosporus) complex in the Northern and Southern Hemispheres
- Association of Sixty-One Non-Synonymous Polymorphisms in Forty-One Hypertension Candidate Genes with Blood Pressure Variation and Hypertension
- Genetic Variations of HSD11B2 in Hypertensive Patients and in the General Population, Six Rare Missense/Frameshift Mutations
- OJ-041 A Common Genetic Variation in the ABCA1 Gene is Associated with Left Ventricular Diastolic Dysfunction in Men with Essential Hypertension(Atherosclerosis, clinical-3 (H) OJ7,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meetin
- A Novel Missense Mutation, F826Y, in the Mineralocorticoid Receptor Gene in Japanese Hypertensives : Its Implications for Clinical Phenotypes
- Contribution of Single Nucleotide Polymorphisms in Protein-Tyrosin Kinase 2 to the Progression of Atherosclerosis in Patients with Essential Hypertension(Hypertension, Clinical 5 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Association of Plasma Platelet Activating Factor-acetylhydrolase (PAF-AH) Gene Polymorphism with Carotid Atherosclerosis in Essential Hypertension(The Frontier of Atherosclerosis Research (IHD), The 69th Annual Scientific Meeting of the Japanese Circulati
- The Thiazide-Sensitive Na^+-Cl^- Cotransporter Gene, C1784T, and Adrenergic Receptor-β3 Gene, T727C, May Be Gene Polymorphisms Susceptible to the Antihypertensive Effect of Thiazide Diuretics
- Six Missense Mutations of the Epithelial Sodium Channel β and γ Subunits in Japanese Hypertensives
- PE-452 Catalogue of Matrix Metalloproteinase Gene Polymorphisms and Susceptibility to Cardiovascular Hypertrophy in Japanese Hypertensive Patients(Hypertension, Clinical 6 (H) : PE77)(Poster Session (English))
- PE-293 Single polymorphism of Lipocalin-type prostaglandin D synthase gene is associated with HDL-cholesterol levels and carotid plaques in hypertensive patients(Heart Failure, Clinical 11 (M) : PE50)(Poster Session (English))
- PE-204 Single Nucleotide Polymorphisms for Determining the Renal Blood Flow in the Patients with Essential Hypertension(Kidney/Renal Circulation 1 (H) : PE35)(Poster Session (English))
- FRS-162 Association of Insulin-Like Growth Factor (IGF)-1 and IGF-1 Receptor Gene Polymorphisms with Cardiac Hypertrophy and Diastolic Function in Essential Hypertension(Hypertension (H) : FRS19)(Featured Research Session (English))
- Conidium morphology of Curvularia geniculata and allied species
- Seed Formation is Affected by Uniconazole, an Inhibitor of Gibberellin Biosynthesis, in a Parthenocarpic Cultivar Severianin of Tomato (Lycopersicon esculentum Mill.)
- Capsinoid Is Biosynthesized from Phenylalanine and Valine in a Non-Pungent Pepper, Capsicum annuum L. cv. CH-19 Sweet
- Hypertension Susceptibility Genes on Chromosome 2p24-p25 in a General Japanese Population(Hypertension, Clinical 6 (H), The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Screening of Chrysanthemum Plants with Strong Resistance to Chrysanthemum Stunt Viroid
- Effects of CH-19 Sweet, a Non-Pungent Cultivar of Red Pepper, in Decreasing the Body Weight and Suppressing Body Fat Accumulation by Sympathetic Nerve Activation in Humans
- Dynamic Model of Dry Matter Distribution and Stabilization in the Number of Buds per Inflorescence by Overnight Supplemental Lighting in Sweet Pea (Lathyrus odoratus L.)
- Effect of Plant Density and Variety on Allometry of Inflorescence Architecture in Gypsophila paniculata L.
- Isolation of basidiomycetous anamorphic yeast-like fungus Meira argovae found on Japanese bamboo
- Species separation in Curvularia "geniculata"group inferred from Brn1 gene sequences
- Indole-3-acetic acid biosynthesis in Aciculosporium take, a causal agent of witches' broom of bamboo
- Dramatic changes in leaf development of the native Capsicum chinense from the Seychelles at temperatures below 24℃
- Polyoxin Resistance of Reddish Brown Laboratory Mutants of Cochliobolus heterostrophus
- Genetic analysis of Cochliobolus heterostrophus polyoxinresistant mutants
- Genetic analyses of Cochliobolus heterostrophus albino mutant with deficiencies at two loci
- Natural infection of Chrysanthemum stunt viroid in dahlia plants
- A Simple and Simultaneous Detection Method for Two Different Viroids Infecting Chrysanthemum by Multiplex Direct RT-PCR
- Evaluation of the Lys198Asn and - 134delA Genetic Polymorphisms of the Endothelin-1 Gene
- Accumulation of anthraquinones in the reddish brown-colored polyoxinresistant mutants of Cochliobolus heterostrophus
- Capsiate, a Nonpungent Capsaicin Analog, Increases Endurance Swimming Capacity of Mice by Stimulation of Vanilloid Receptors
- CH-19 Sweet, a Non-Pungent Cultivar of Red Pepper, Increased Body Temperature and Oxygen Consumption in Humans(Food & Nutrition Science)
- CH-19 Sweet, Nonpungent Cultivar of Red Pepper, Increased Body Temperature in Mice with Vanilloid Receptors Stimulation by Capsiate
- FRS-160 Single Nucleotide Polymorphisms Relating to Low-Renin Essential Hypertension(Hypertension (H) : FRS19)(Featured Research Session (English))
- Polymorphism of Na+/Ca2+ Exchanger Gene and AntihypertensiveResponse to Calcium Channel Blockers in Patients with Essential Hypertension
- Two new species of Amanita from Japan
- Amanita concentrica : a new species in Amanita section Amanita from Japan
- Amanita sinensis, new to Japan and Nepal
- Molecular phylogeny of Japanese Amanita species based on nucleotide sequences of the internal transcribed spacer region of nuclear ribosomal DNA
- Phylogenetic study of clavicipitaceous fungi using acetaldehyde dehydrogenase gene sequences
- A PCR-based method for mating type determination in Cochliobolus heterostrophus
- Susceptible Gene Polymorphisms to the Antihypertensive Effect of Angiotensin Converting Enzyme Inhibitors
- Association between Polymorphisms of Kallikrein Gene and Resistant to Anti-Hypertensive Therapy
- Susceptible Gene Polymorphisms to the Antihypertensive Effect of Thiazide Diuretics
- Effects of CH-19 Sweet, a Non-Pungent Cultivar of Red Pepper, on Sympathetic Nervous Activity, Body Temperature, Heart Rate, and Blood Pressure in Humans
- Capsinoids, Non-Pungent Capsaicin Analogs, Reduce Body Fat Accumulation without Weight Rebound unlike Dietary Restriction in Mice
- Induced resistance against Fusarium diseases of Cymbidium species by weakly virulent strain HPF-1 (Fusarium sp.)
- An Alternative Fast and Convenient Genotyping Method for the Screening of Angiotensin Converting Enzyme Gene Polymorphisms
- Coprinopsis austrophlyctidospora sp. nov., an agaric ammonia fungus from Southern Hemisphere plantations and natural forests
- Identification and Sterilization of Epiphytic Bacterial Flora near Hydrangea Shoot Apical Meristems
- Simple transformation of the rice false smut fungus Villosiclava virens by electroporation of intact conidia
- Leaf Primordia-Free Shoot Apical Meristem Culture : A New Method for Production of Viroid-Free Plants
- Molecular organization of the mating type (Mat) locus of Exserohilum monoceras (Setosphaeria monoceras), a bioherbicide agent for Echinochloa weeds
- Defense response of a pepper cultivar cv. Sy-2 is induced at temperatures below 24℃
- Teleomorph formation of Setosphaeria monoceras, a perfect state of Exserohilum monoceras, by Japanese isolates