Functional Characterization of Ergothioneine Transport by Rat Organic Cation/Carnitine Transporter Octn1 (slc22a4)(Biopharmacy)
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概要
- 論文の詳細を見る
It has been reported that organic cation/carnitine transporter 1 (OCTN1) is associated with rheumatoid arthritis and Crohn's disease. Additionally, we reported that OCTN1 is expressed in hematopoietic cells, and is associated with proliferation and differentiation of erythroid cells. However, physiological role of OCTN1 is still unclear. Ergothioneine, an anti-oxidant, was recently reported to be a good substrate of human OCTN1. However, the transport characteristics of ergothioneine in rat remains to be clarified. The present study, is to further investigate the role of rat Octn1 on transport of ergothioneine in rat Octn1 transfected cells and natively expressing cell line PC12 derived from rat adrenal pheochromocytoma. [^3H]Ergothioneine uptake by rat Octn1 stably transfected HEK293 cells was saturable, sodium dependent with 1:1 stoichiometry of ergothioneine, and pH dependent. Since ergothioneine was reported to presumably play a protective role against oxidative stress-induced apoptosis in PC12 cells, its transport in this cell line was investigated. The expression of rat Octn1 and a saturable and Na^+-dependent transport of ergothioneine were observed in PC 12 cells, suggesting that ergothioneine transport in this cell line may be mediated by rat Octn1. These findings suggested that rat Octn1 may act as a survival factor by taking up ergothioneine to suppress oxidative stress in this cell line. In conclusion, functional characteristics of ergothioneine transport by rat Octn1 is similar to that of human OCTN1 and it is suggested that rat Octn1 is important by transporting anti-oxidant ergothioneine in PC12 cells, though its role in vivo is to be investigated.
- 公益社団法人日本薬学会の論文
- 2008-08-01
著者
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YOSHIDA Kenji
Department of Electronic Information Systems, Shibaura Institute of Technology
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Tamai Ikumi
Dep. Of Membrane Transport And Biopharmaceutics Fac. Of Pharmacy Inst. Of Medical Pharmaceutical And
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Tamai Ikumi
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmacy Institute Of Medical Pharm
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Tamai Ikumi
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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MAEDA Tomoji
Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmaceutical Sciences, Tokyo Uni
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YABUUCHI Hikaru
GenoMembrane, Inc.
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Maeda Tomoji
Dep. Of Molecular Biopharmaceutics Fac. Of Pharmaceutical Sciences Tokyo Univ. Of Sci.
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Maeda Tomoji
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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Yabuuchi Hikaru
Genomembrane Inc.
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NAKAMURA Toshimichi
Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Ph
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Nakamura Toshimichi
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmacy Institute Of Medical Pharm
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Maeda Tomoji
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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Yoshida Kenji
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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Yoshida Kenji
Department Of Chemistry Faculty Of Science Ehime University
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Yoshida Kenji
Department of Biochemistry, Kinki University School of Medicine
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