Role of Polyamines Derived from Arginine in Differentiation and Proliferation of Human Blood Cells(Molecular and Cell Biology)
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概要
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L-Arginine is a precursor of polyamine, nitric oxide (NO), creatine, and agmatine and is essential for the differentiation and proliferation of blood cells, although the precise biological role of L-arginine is unclear. We have recently reported that the depletion of L-arginine in cultured medium prevented both proliferation and differentiation of blood cells (Shima et al., Blood First Edition Paper, October 6, 2005; DOI 10.1182). Since one of metabolic products of L-arginine in the cells is polyamine that associates with cell differentiation and proliferation, the effects of L-arginine on the human K562 cell line and human cord blood-derived CD34 positive cells were investigated by focusing on polyamines such as putrescine, spermidine, and spermine in the present study. When polyamines were added to the culture medium in the absence of L-arginine, the cells did not grow or differentiate well. However, when intracellular polyamines were depleted using ornithine decarboxylase inhibitor, alpha-diflu-oromethylornithine (DFMO), the proliferation and differentiation of K562 cells to erythrocytes were reduced even in the presence of L-arginine. Moreover, in the presence of DFMO, cell differentiation and proliferation were recovered by the addition of putrescine or spermidine in the presence of L-arginine. Accordingly, it was demonstrated that polyamines are essential for the proliferation and differentiation of the blood cells as the metabolites of L-arginine and the externally added polyamines are also effective by being taken up through polyamine transporter.
- 公益社団法人日本薬学会の論文
- 2006-02-01
著者
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AIZAWA Shin
Department of Functional Morphology, Nihon University School of Medicine
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Aikawa Shingo
日本大学 医学部内科
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Aizawa Shin
Department Of Anatomy Nihon University School Of Medicine
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Tamai Ikumi
Dep. Of Membrane Transport And Biopharmaceutics Fac. Of Pharmacy Inst. Of Medical Pharmaceutical And
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Tamai Ikumi
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmacy Institute Of Medical Pharm
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Tamai Ikumi
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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TSUBOI Isao
Department of Anatomy, Nihon University School of Medicine
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MAEDA Tomoji
Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmaceutical Sciences, Tokyo Uni
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WAKASAWA Takeru
Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmaceutical Sciences, Tokyo Uni
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Tsuboi Isao
Department Of Anatomy Nihon University School Of Medicine
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Maeda Tomoji
Dep. Of Molecular Biopharmaceutics Fac. Of Pharmaceutical Sciences Tokyo Univ. Of Sci.
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Maeda Tomoji
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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Aikawa Shingo
Department Of Anatomy Nihon University School Of Medicine
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Wakasawa Takeru
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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SHIMA Yoichiro
Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmaceutical Sciences, Tokyo Uni
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Shima Yoichiro
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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Maeda Tomoji
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
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AIZAWA Shin
Department of Anatomy, Nihon University School of Medicine
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