Involvement of Choline Transporter-Like Proteins, CTL1 and CTL2, in Glucocorticoid-Induced Acceleration of Phosphatidylcholine Synthesis via Increased Choline Uptake
スポンサーリンク
概要
- 論文の詳細を見る
Phosphatidylcholine (PC) production is accelerated by glucocorticoid, such as dexamethasone (DEX), which enhances fetal lung maturation, promotes differentiation of alveolar type II (ATII) cells, and increases production of both lipid and protein components of lung surfactant. We previously demonstrated that inhibition of choline uptake by ATII cells leads to a decrease of PC synthesis. Since choline uptake may play a critical role in PC production and lung surfactant homeostasis for normal breathing, it is of interest to characterize transporters controlling the disposition of choline in ATII cells. Therefore, we studied the gene regulation and activity of choline transporters in A549 cells, a human ATII cell line. A549 cells were exposed to DEX for 24 h, and mRNA expression levels of choline transporters-like protein 1, (CTL1) and CTL2, were measured using real-time reverse transcription polymerase chain reaction. CTL1 and CTL2 mRNAs were strongly induced by DEX treatment of A549 cells, and the DEX-treated cells showed a significant increase in initial uptake rate of [3H]choline, which was assessed under ATP-depleted conditions to block the influence of consumption of choline by choline kinase. Transfection of A549 cells with either CTL1- or CTL2-small interfering RNAs significantly decreased [3H]choline uptake. In conclusion, choline transport in A549 cells is increased by treatment with DEX, and the increase is mediated by induction of functional choline transporters CTL1 and CTL2.
著者
-
Tamai Ikumi
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
-
Nakanishi Takeo
Department Of Animal Husbandry Faculty Of Agriculture Tohoku University
-
Maeda Tomoji
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmaceutical Sciences Tokyo Unive
-
Nakamura Toshimichi
Department Of Membrane Transport And Pharmacokinetics Faculty Of Pharmacy Institute Of Medical Pharm
-
Fujiwara Ryohei
Faculty of Pharmaceutical Sciences, Tokyo University of Science
-
Ishiguro Naoki
Faculty of Pharmaceutical Sciences, Tokyo University of Science
-
Oyabu Masanobu
Faculty of Pharmaceutical Sciences, Tokyo University of Science
-
Shirasaka Yoshiyuki
Department of Membrane Transport and Pharmacokinetics, Faculty of Pharmacy, Institute of Medical, Ph
-
Ishiguro Naoki
Faculty of Medical Technology, School of Health Sciences, Fujita Health University
関連論文
- Regulation of Octn2 Transporter (SLC22A5) by Peroxisome Proliferator Activated Receptor Alpha(Biopharmacy)
- Decalcification of Calcium Polycarbophil in Rats
- Effect of Polycarbophil on the Absorption of Nutrients
- Design and Synthesis of Peptides Passing through the Blood-Brain Barrier
- Transient Behavior of Voltage Holding Ratio in Nematic Liquid Crystal Cells(Optics and Quantum Electronics)
- In Situ and in Vitro Evidence for Stereoselective and Carrier-Mediated Transport of Monocarboxylic Acids across Intestinal Epithelial Tissue
- Effect of Glucocorticoid Receptor Ligand Dexamethasone on the Expression of Organic Cation Transporter in Rat Liver
- Characterization of Nucleobase Transport by Mouse Sertoli Cell Line TM4(Biopharmacy)
- Functional Characterization of Ergothioneine Transport by Rat Organic Cation/Carnitine Transporter Octn1 (slc22a4)(Biopharmacy)
- Functional Characterization of Ergothioneine Transport by Rat Organic Cation/Carnitine Transporter Octn1 (slc22a4)
- Involvement of Bile Salt Export Pump in Flutamide-Induced Cholestatic Hepatitis(Biopharmacy)
- Muscle Distribution of Antimicrobial Agents after a Single Intravenous Administration to Rats
- Changes of Differentiation and Proliferation in K562 Cells with Various Levels of Knockdown of Cationic Amino Acid Transporter 1
- Role of Polyamines Derived from Arginine in Differentiation and Proliferation of Human Blood Cells(Molecular and Cell Biology)
- Involvement of Choline Transporter-Like Proteins, CTL1 and CTL2, in Glucocorticoid-Induced Acceleration of Phosphatidylcholine Synthesis via Increased Choline Uptake
- DISTRIBUTION OF PHOSPHORUS IN MILK AND ITS CHANGES BEFORE AND AFTER SOURING
- STUDIES ON THE IMPROVEMENT OF BUTTER-MAKING PROCEDURE I. VITAMIN A POTENCY OF JAPANESE BUTTER
- CHEMICAL COMPOSITION OF EWES' MILK AT DIFFERENT STAGES OF LACTATION PERIOD
- THE ISOLATION AND IDENTIFICATION OF GLUCOSE IN RIPE CHEESE
- SENSORY PANEL TESTING OF THE CHEESE RIPENED WITH ASPERGILLUS ORYZAE-CHOSEN B
- STUDIES OF ANTIOXYGENIC ACTION OF VITAMIN C ON THE CONSTITUENTS OF DRIED WHOLE MILK I. EFFECT OF HYDROQUINONE AND cis-ISOSAFROEUGENOL
- STUDIES OF ANTIOXYGENIC ACTION OF VITAMIN C ON THE CONSTITUENTS OF DRIED WHOLE MILK II. EFFECT OF MUCH ADDED VITAMIN C