35 高等植物はモノテルペノイドでアポトーシスを引き起こす : その初期過程の解明(口頭発表の部)

概要

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To investigate the defense mechanism of plant cells against chemical stress, we have examined the defense reaction of plant cells against exogenous monoterpenoids. We have screened various isoprenoids to search for inducers of apoptosis in the cultured cells of several plants such as tabacco, chamomile, celery, soybean and liverwort. These studies have revealed that geraniol has the most potent apoptosis-inducing activity, as judged from DNA fragmentation in the cells. The apoptosis-inducing activity of geraniol is concentration- and time-dependent. Morphologically, the nuclei of the cells which have been treated with 5mM of geraniol were condensed and fragmented. On the other hand, additoion of 1ppm of N^6-(Δ^2-isopentenyl) adenosine and kinetin prevented the geraniol-induced DNA fragmentation in the cells. Furthermore, cycloheximide and actinomycin D, inhibitors of protein and RNA syntheses, respectively, did not suppress the apoptosis of the plant cells. This result suggests that the apoptotic process induced by geraniol does not require the synthesis of new proteins. The cytokinin inhibition mechanism of the apoptosis induced by geraniol will be discussed.
天然有機化合物討論会の論文
1999-09-01