1 下等植物は高等植物からどのようにして身を守るか : ゼニゴケ培養細胞における生体防御機構(口頭発表の部)

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To clarify a defense mechanism of liverworts against the higher plants, we examined defense reaction of the cultured cells of M. polymorpha against metabolites of higher plants, e.g. monoterpenoids. When bornyl acetate (1) was added into the cultured cells of M. polymorpha as a stress substrate, 4-[2-(3-hydroxyphenyl)ethyl]phenol (2) and 4-hydroxy-2,3-dimethyl-2-nonen-4-olide (3) were secreted from the cells as the allelochemicals. Especially, 3 induced the complete death of the cultured cells of Nicotiana tabacum. To elucidate the biosyntheses of 3, feeding experiments of [1- ^<14>C]acetic acid and [4,5-^3H] isoleucine into 3 were carried out. [1-^<14>C]Acetic acid was incorporated into 3 in 0.11 %. On the other hand, [4,5-^3H]isoleucine was incorporated into 3 in 0.016 % via angelic acid. These results suggested that 3 is biosynthesized from angelic acid and caproic acid, which were produced from isoleucine and acetic acid, respectively. Nextly, a transduction mechanism of the signal against the bornyl acetate-stimulation in the cultured cells of M. polymorpha was examined. Addition of bornyl acetate (1) to the cultured cells of M. polymorpha caused a rapid increase in the intracellular level of cAMP. Furthermore, the Sp-cAMPS, which is a cAMP agonist, activated the H_2O_2 formation and the cAMP antagonist (Rp-cAMPS) inhibited the H_2O_2 formation. These results suggest that a defense reaction against monoterpemoids exists in liverwort.
天然有機化合物討論会の論文
1996-09-02

1 下等植物は高等植物からどのようにして身を守るか : ゼニゴケ培養細胞における生体防御機構(口頭発表の部)

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